Yeast Synthetic Drop-out Medium Supplements
SIGMA/Y2146 - without histidine, leucine, and tryptophan
Synonym: Synthetic yeast drop-out mix; Yeast Synthetic Drop-out Media Supplements; Yeast media; amino acid dropout supplements
MDL Number: MFCD01095679
Product Type: Chemical
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| application(s) | microbiology |
| form | powder |
| Quality Level | 200  |
| storage temp. | room temp |
| technique(s) | transformation: suitable |
| Application: | The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. |
| Application: | Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants. |
| Other Notes: | Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids. |
| Preparation Note: | Dissolve 1.46 g of supplement per liter of medium. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106212 |