2x YT medium
SIGMA/Y2377 - Powder microbial growth medium
Synonym: 2x Yeast Extract Tryptone medium; 2xTY medium; 2xYT Medium; 2xYT media
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: Y2377-250G
| application(s) | food and beverages microbiology |
| form | powder |
| grade | Molecular Biology |
| pH | 6.8-7.2(3.1% solution) |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | 2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B. |
| Features and Benefits: | 2xYT powder provides:• Standard formulation • An inexpensive alternative to liquid |
| General description: | 2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. |
| Other Notes: | 16 g/L Tryptone 10 g/L Yeast Extract 5 g/L NaCl |
| Packaging: | 250 g in poly bottle |
| Preparation Note: | 1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water. 2. Heat to boiling while stirring to dissolve all ingredients completely. 3. Autoclave for 15 minutes at 121 °C. |
| Preparation Note: | Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106225 |