2x YT medium
SIGMA/Y2377 - Powder microbial growth medium
Synonym: 2xYT Medium; 2xYT media
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: Y2377-250G
| application(s) | food and beverages |
| form | powder |
| grade | for molecular biology |
| pH | 6.8-7.2(3.1% solution) |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms |
| Application: | 2x YT medium has been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B. |
| Application: | Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added. |
| Components: | 16 g/L Tryptone 10 g/L Yeast Extract 5 g/L NaCl |
| Features and Benefits: | 2xYT powder provides: • Standard formulation • An inexpecnsive alternative to liquid |
| General description: | 2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. |
| Packaging: | 250 g in poly bottle |
| Reconstitution: | Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12352200 |