2xYT Medium
SIGMA/Y2627 - EZMix Powder microbial growth medium
Product Type: Chemical
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| application(s) | agriculture food and beverages |
| composition | Inert binder (EZMix only), 0.6 g/L |
| NaCl, 5 g/L | |
| Tryptone (pancreatic digest of casein), 16 g/L | |
| Yeast extract, 10 g/L | |
| description | nutrient rich, quick dissolve, dust free formulation |
| form | powder |
| grade | Molecular Biology |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms |
| Application: | Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added. |
| Features and Benefits: | 2xYT EZMix powder provides: • Granulated, dust-free format for safer handling and faster mixing • Easy scale-up using larger package size • Standard formulation |
| General description: | 2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. |
| Packaging: | 1 kg in poly bottle |
| Preparation Note: | Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106200 |