2xYT Medium
SIGMA/Y2627 - EZMix™ Powder microbial growth medium
Product Type: Chemical
application(s) | agriculture food and beverages |
composition | Inert binder (EZMix only), 0.6 g/L |
NaCl, 5 g/L | |
Tryptone (pancreatic digest of casein), 16 g/L | |
Yeast extract, 10 g/L | |
description | nutrient rich, quick dissolve, dust free formulation |
form | powder |
grade | for molecular biology |
sterility | non-sterile |
storage temp. | room temp |
suitability | nonselective for Escherichia coli |
nonselective for coliforms |
Application: | Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added. |
Features and Benefits: | 2xYT EZMix™ powder provides: • Granulated, dust-free format for safer handling and faster mixing • Easy scale-up using larger package size • Standard formulation |
General description: | 2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. |
Legal Information: | EZMix is a trademark of Merck KGaA, Darmstadt, Germany |
Packaging: | 1 kg in poly bottle |
Reconstitution: | Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | room temp |
UNSPSC | 12352200 |