Yeast Carbon Base
SIGMA/Y3627 - suitable for microbiology, NutriSelect® Basic
Product Type: Chemical
| application(s) | clinical testing food and beverages life science and biopharma |
| microbiology | |
| form | powder |
| manufacturer/tradename | NutriSelect® Basic |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | 2-8°C |
| suitability | nonselective for Candida spp. |
| nonselective for Pichia spp. | |
| nonselective for Saccharomyces spp. | |
| nonselective for Zygosaccharomyces spp. | |
| technique(s) | microbe id | utilization test: suitable |
| microbiological culture: suitable |
| Application: | Recommended for use in classification of yeasts on the basis of their ability to assimilate nitrogen compounds. |
| Components: | Ingredients (g/L) Dextrose, 10.00 Biotin, 0.000002 Calcium pantothenate, 0.0004 Folic acid, 0.000002 Inositol, 0.002 Niacin, 0.0004 p-Amino benzoic acid, 0.0002 Pyridoxine hydrochloride, 0.0004 Riboflavin (Vitamin B2), 0.0002 Thiamine hydrochloride, 0.0004 Boric acid, 0.0005 Coppers sulfate, 0.00004 Potassium iodide, 0.0001 Ferric chloride, 0.0002 Manganese sulfate, 0.0004 Sodium molybdate, 0.0002 Zinc sulfate, 0.0004 Monopotassium phosphate, 1.00 Magnesium sulfate, 0.50 Sodium chloride, 0.10 Calcium chloride, 0.10 |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | A. Nitrogen Assimilation test Prepare the broth base at 10 x concentration by dissolving 11.7 grams of Yeast Carbon Base in 100 mL of distilled water. Add the appropriate nitrogen source. Warm if necessary to dissolve the medium completely. Sterilize by filtration. B. Detection of yeasts, other than Saccharomyces cerevisae Dissolve 2.35 grams of Yeast Carbon Base in 100 mL of distilled water. C. Detection of wild yeasts in beer and other brewing components Add 0.33 g of ammonium sulphate (Catalog No. A2939) and 4 g of bacteriological agar (Catalog No. A5306) to base B. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
| Risk Statements | 52/53 |
| Safety Statements | 61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41171606 |