Anti-YAP1 (C-terminal) antibody produced in rabbit
SIGMA/Y4770 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-YAP; Anti-YAP2; Anti-YAP65; Anti-Yes-associated protein 1, 65kDa
MDL Number: MFCD04118707
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-YAP1 (C-terminal) antibody produced in Rabbit (Cat. No. Y4770) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing YAP1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:2,000.
Immunoblotting
Whole cell lysates of HEK293 cells co-transfected with human c-Abl and human YAP1 (Lanes 1,3) or untransfected HEK-293T (Lane 2) were separated on SDS-PAGE and blotted with Anti-YAP1 (C-terminal) (Cat. No. Y4770). The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
Lanes
1,2: Antibody dilution 1:2,000
3: Antibody dilution 1:2,000 + Inhibition with YAP1 immunizing peptide (human 442-454) (20 μg/mL)
Whole cell lysates of HEK293 cells co-transfected with human c-Abl and human YAP1 (Lanes 1,3) or untransfected HEK-293T (Lane 2) were separated on SDS-PAGE and blotted with Anti-YAP1 (C-terminal) (Cat. No. Y4770). The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
Lanes
1,2: Antibody dilution 1:2,000
3: Antibody dilution 1:2,000 + Inhibition with YAP1 immunizing peptide (human 442-454) (20 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~65 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and c-Abl |
| UniProt accession no. | P46937  |
| Application: | Anti-YAP1 (C-terminal) antibody is suitable for use in western blot (.5-1 μg/mL) using HEK-293T cells co-transfected with human YAP1 and c-Abl. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunoprecipitation (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | YAP1 (Yes-associated protein 1) induces p73-dependent apoptosis through the specific and selective co-activation of p53AIP1, an apoptotic p73 target gene. It stabilizes p73 by preventing Itch-mediated ubiquitination of p73. Upon phosphorylation by Akt at Ser127, 14-3-3 is recruited and stimulates YAP1 localization to the cytoplasm, resulting in loss of co-activator function in the nucleus. Inhibition of Akt potentiates the nuclear re-localization of YAP1 to promote apoptosis by p73. |
| Biochem/physiol Actions: | YAP1 is a WW domain containing protein that functions as a transcriptional co-activator . It may also regulate p73 gene function in response to DNA damage. YAP1 is known to associate with a wide range of transcription factors . Alterations in YAP1 have been associated with tumor development . |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | YAP1 (Yes-associated protein 1, YAP, YAP2, YAP65) is a modular adaptor protein with multiple protein interaction domains, that is based on its interaction with Src-family tyrosine kinase c-Yes. It has a SH3-binding motif, proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 also contains a 14-3-3 interacting motif. |
| Immunogen: | synthetic peptide corresponding to amino acids 442-454 located at the C-terminus of human YAP1. The sequence is identical in mouse, bovine, and dog YAP1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Specificity: | Anti-YAP1 (C-terminal) antibody is specific for human YAP1. The product is expected to react with mouse, bovine, and dog YAP1. In immunoblotting, detection of the YAP1 band is specifically inhibited with the immunizing peptide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |