Yeast Transformation Kit
SIGMA/YEAST1 - reagents for introducing plasmid DNA into yeast
Synonym: lithium acetate yeast transformation
Product Type: Chemical
grade | for molecular biology |
Quality Level | 200 ![]() |
shipped in | dry ice |
storage temp. | −20°C |
usage | kit sufficient for >100 standard transformations |
Application: | Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range. |
Components: | The Yeast Transformation Kit contains: • Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA • Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA • Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml • Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg • Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g |
Features and Benefits: | • Easy and ready-to-use • Requires as little as 10 ng of plasmid DNA • Flexibility for any strain of yeast • Sufficient for over 100 standard transformations |
General description: | Sigma’s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method. |
Principle: | Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG). |
Symbol | ![]() |
Signal word | Warning |
Hazard statements | H373 |
Precautionary statements | P260 - P314 - P501 |
Hazard Codes | Xi |
Risk Statements | 41 |
Safety Statements | 26-39 |
RIDADR | UN 3316 9 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352200 |
Components | Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1 mL; Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g |