Yeast Transformation Kit
SIGMA/YEAST1 - reagents for introducing plasmid DNA into yeast
Synonym: lithium acetate yeast transformation
Product Type: Chemical
The Yeast Transformation kit was used to prepare competent cells from Saccharomyces cerevisiae strain YPH858 and to transform the cells with increasing amounts of plasmid DNA (pRS316). After transformation, the yeast were diluted and plated on yea
| grade | Molecular Biology |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | transformation: suitable |
| usage | 1 kit sufficient for >100 standard transformations |
| Application: | Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range. |
| Biochem/physiol Actions: | Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG). |
| Features and Benefits: | • Easy and ready-to-use • Requires as little as 10 ng of plasmid DNA • Flexibility for any strain of yeast • Sufficient for over 100 standard transformations |
| General description: | Sigma’s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method. |
| Other Notes: | The Yeast Transformation Kit contains: • Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA • Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA • Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml • Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg • Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H373 |
| Precautionary statements | P260 - P314 - P501 |
| Hazard Codes | Xi |
| Risk Statements | 41 |
| Safety Statements | 26-39 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1 mL; Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g |