Monoclonal Anti-ZAP-70 antibody produced in mouse
SIGMA/Z0627 - ~1.5 mg/mL, clone 1E7.2, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD00803577
Product Type: Chemical
Immunoblotting
Cell extract from Jurkat cells (Human Breast Carcinoma) was separated by SDS-PAGE, blotted with (Lane 1) or without (Lane 2) Monoclonal Anti-ZAP70 (Cat. No. Z0627) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304).
Cell extract from Jurkat cells (Human Breast Carcinoma) was separated by SDS-PAGE, blotted with (Lane 1) or without (Lane 2) Monoclonal Anti-ZAP70 (Cat. No. Z0627) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1E7.2, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~70 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1.0 μg/mL using Jurkat cell extract | |
| UniProt accession no. | P43403  |
| Application: | Monoclonal Anti-ZAP-70 antibody has been used in • enzyme linked immunosorbent assay (ELISA) • immunoblottin • flow cytometry • immunoprecipitation • immunocytochemistry |
| Application: | Monoclonal Anti-ZAP-70 antibody is suitable for use in western blot (0.5-1.0 μg/mL using Jurkat cell extract), flow cytometry, immunocytochemistry, immunoprecipitation, microarray and indirect ELISA. |
| Biochem/physiol Actions: | ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the zeta chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling . Lack of ZAP-70 leads to an absence of antigen-specific response and also causes impaired T cell differentiation . Anti-ZAP-70 antibody is specific for human and mouse ZAP-70 (approx. 70 kDa). |
| Biochem/physiol Actions: | Zeta-chain-associated protein kinase70 (ZAP-70) is required for T-cell activation and is activated by(Tyrosine protein kinase) Lck-mediated phosphorylation of its tyrosine residues. Mutation of the gene that creates a defective ZAP-70 results in severe combined immunodeficiency syndrome (SCID). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ZAP-70 (mouse IgG1 isotype) is derived from the hybridoma 1E7.2 produced by the fusion of mouse myeloma cells and splenocytes from mice. ZAP-70 is a protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells. |
| Immunogen: | peptide corresponding to amino acids 282-307 of human ZAP-70. |
| Immunogen: | The immunogen sequence is conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |