ZAP-70 Tyrosine Kinase human
SIGMA/Z2126 - ≥90% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells (Histidine tagged), buffered aqueous glycerol solution, ≥150 U/mg
MDL Number: MFCD03459122
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | 70 kDa |
| Quality Level | 200  |
| recombinant | expressed in baculovirus infected insect cells (Histidine tagged) |
| shipped in | dry ice |
| specific activity | ≥150 U/mg |
| storage temp. | −70°C |
| UniProt accession no. | P43403  |
| Application: | Human ZAP-70 Tyrosine Kinase was used for mapping the phosphorylation sites on LAT (linker for activation of T cells) for investigating the mechanism of activation of signalling proteins in T cells. |
| Biochem/physiol Actions: | ZAP-70 is protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells. It is required for T cell activation. It is activated by Lck-mediated phosphorylation of its tyrosine residues. |
| Biochem/physiol Actions: | ZAP-70 Tyrosine Kinase is a protein belongs to Syk family and is essential for T cell activation. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. Additionally, it also plays a pivotal role in LFA-1-dependent T Cell Migration. |
| Other Notes: | One unit will phosphorylate 1 nmol polyglutamic acid:tyrosine per min at 37 °C at pH 7.0. |
| Physical form: | Solution in 25 mM HEPES, pH 7.5, containing 150 mM NaCl, 0.5 mM DTT, and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥150 U/mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |