Anti-SARS-CoV-1/2 S protein CR3022 Antibody ZooMAb® Human Monoclonal
SIGMA/ZHU1077 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Western Blotting Enhanced Validation - Recombinant Antibody Technology Recombinant extracellular domain of SARS-COV-2 was probed with Cat. No. ZHU1077, Anti-SARS-CoV-1/2 S protein CR3022, ZooMAb® Human Monoclonal (1:1,000 dilution). Proteins were visualized using a Goat Anti-Rabbit IgG1 secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SARS-CoV-1/2 S protein CR3022 (~139 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 mg/mL of recombinant RBD of SARS-CoV-2 spike protein (Run 1) or recombinant ECD of spike protein (Run 2) or non-specific nucleocapsid protein (Run 3) were analyzed by affinity binding assay using Cat. No. ZHU1077, Anti-SARS-CoV-1/2 S protein CR3022, ZooMAb® Human Monoclonal. The binding of this antibody to RBD displayed a KD of 4.4 x 10-7 whereas binding to ECD showed a KD of 2.4 x 10-7 and binding to nucleocapsid protein displayed a KD of 1.1 x 10-4.
ELISA Enhanced Validation - Recombinant Antibody Technology SARS-CoV-2 spike protein and the receptor binding domain of spike protein were analyzed using multiple dilutions of Cat. No. ZHU1077, Anti-SARS-CoV-1/2 S protein CR3022, ZooMAb® Human Monoclonal. Plate was quoted with 100 mL of 1 mg/mL of spike protein, receptor binding domain, or BSA and proteins were detected using HRP conjugated Goat Anti-Human IgG (1:5,000 dilution).
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million CHO cells expressing RBD was performed using of Cat. No. ZHU1077, Anti-SARS-CoV-1/2 S protein CR3022, ZooMAb® Human Monoclonal (Blue histogram) or the equivalent amount of a of Human Recombinant IgG1 isotype control (Red histogram), followed by a PE-conjugated IgG1 secondary antibody.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million spike or untransfected was performed using of Cat. No. ZHU1077, Anti-SARS-CoV-1/2 S protein CR3022, ZooMAb® Human Monoclonal (Blue histogram) or the equivalent amount of a of Human Recombinant IgG1 isotype control (Red histogram), followed by a PE-conjugated IgG1 secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | human |
| clone | CR3022, recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1 |
| mol wt | calculated mol wt 139.13 kDa |
| calculated mol wt 141.18 kDa | |
| observed mol wt ~130 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | SARS virus |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| enzyme immunoassay: suitable | |
| flow cytometry: suitable | |
| neutralization: suitable | |
| western blot: suitable |
| Application: | Quality Control Testing Western Blot Analysis: A 1:1,000 dilution of this antibody detected Rac1 in Rat Brain Microsomes. Tested applications Western Blot Analysis: A 1:1,000 dilution from a representative lot detected Rac1 in A431, HeLa, and NIH3T3 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:100 silution from a representative lot detected Rac1 in human esophagus tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rac1 in HeLa, A431, HUVEC , and NIH 3T3 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-SARS-CoV-1/2 S protein CR3022, ZooMAb, Cat. No. ZHU1077, is a human recombinant monoclonal antibody that detects Spike protein from SARS-CoV-1 and -2 and tested for use in Affinity Binding Assay, ELISA, Flow Cytometry, Neutralizing, and Western Blot |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Data presented are the full extent of available current product information and are provided as-is. This product has not been tested or verified in any additional applications, sample types, including clinical and/or patient samples. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested suitable applications and conditions presented in our product information, and is not extended to publications. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Recombinant fragment corresponding to the extracellular domain from SARS-CoV virus spike protein. (CR3022 is an antibody that was originally derived from a SARS-CoV-1-infected patient). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | CR3022 is a ZooMAb human recombinant monoclonal antibody expressed in HEK293 cells that specifically detects SARS-CoV-1/2 Spike protein. It targets an epitope within the N-terminal half, S1 domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | SARS-CoV-1/2 Spike glycoprotein (UniProt: P59594/P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S gene (Gene ID: 1489668/43740568) in SARS-CoV-1/2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 protein, which is reported to be well conserved, is responsible for membrane fusion. CR3022 binds tightly to RBD of the SARS-CoV-2 spike protein at a site different to that used by the receptor. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Huo, J., et al. (2020). Cell Host & Microbe 28(3); 445-454; Ou, X., et al. (2020). Nat. Commun. 11(1); 1620; Shang, J., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(21); 11727-11734). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |