Anti-Actin Antibody, clone C4 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1004 - recombinant, expressed in HEK 293 cells
Synonym: Smooth muscle actin
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat testis (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1004, Anti-Actin, clone C4 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in seminiferous tubules and Leydig cells of rat testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat testis (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1004, Anti-Actin, clone C4 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in seminiferous tubules and Leydig cells of rat testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat testis (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1004, Anti-Actin, clone C4 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in seminiferous tubules and Leydig cells of rat testis tissue sections.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat testis tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1004, Anti-Actin, clone C4 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat testis tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1004, Anti-Actin, clone C4 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat testis tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1004, Anti-Actin, clone C4 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from HEK293 (Lane 1), HeLa (Lane 2), C6 (Lane 3), and NIH3T3 (Lane 4) cells were probed with Cat. No. ZMS1004, Anti-Actin, clone C4 ZooMAb® Mouse Monoclonal (1:10,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG1 secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Actin (~40 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | C4, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1 |
| mol wt | calculated mol wt 42.05 kDa |
| observed mol wt ~40 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | all |
| storage temp. | 2-8°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P68139  |
| Application: | Anti-Actin, clone C4 ZooMAb, Cat. No. ZMS1004, is a recombinant Mouse monoclonal antibody that targets Actin and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Actin in HEK293, HeLa, and NIH3T3 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Actin in rat testis tissue sections. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Actin in rat testis tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Purified actin from chicken gizzard. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone C4 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Actin in most vertebrate species. It targets a conserved epitope within 20 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Actin (UniProt: P68139) is encoded by the ACTA1 (also known as ACTA) gene (Gene ID: 421534) in chicken. Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. Actin cytoskeleton is also involved in the morphogenesis of dendritic spines and is reported to be essential for spine structural stabilization that maintains long-term memory. Clone C4 is prepared as an antibody to chicken gizzard muscles actin, however, it reacts with actins from all vertebrates. It recognizes all six known vertebrate actins and binds to a highly conserved region of actin. This antibody reacts with both globular (G) and filamentous (F) forms of actin and does not interfere with actin polymerization to form filaments. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lessard, DL. (1988). Cell Motil. Cytoskeleton. 10(3); 349-362; Basu, S., and Lamprecht, R. (2018). Front. Mol. Neurosci. 11; 143).. |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |