Anti-GluR1 Antibody, clone RH95 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1007 - recombinant, expressed in HEK 293 cells
Synonym: AMPA-selective glutamate receptor 1; GluA1; GluR-1; GluR-A; GluR-K1; Glutamate receptor 1; Glutamate receptor ionotropic AMPA 1
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons of rat cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons of rat cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons of rat cerebral cortex tissue sections.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat brain tissue sections was performed using a 1:1,000 dilution of Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat brain tissue sections was performed using a 1:1,000 dilution of Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat brain tissue sections was performed using a 1:1,000 dilution of Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of rat E18 cortical cells was performed using a 1:100 dilution of Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the ER, cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of rat E18 cortical cells was performed using a 1:100 dilution of Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the ER, cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of rat E18 cortical cells was performed using a 1:100 dilution of Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the ER, cell membrane.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Mouse brain (Lane 1) and Rat brain (Lane 2) tissue lysates were probed with Cat. No. ZMS1007, Anti-GluR1, clone RH95 ZooMAb® Mouse Monoclonal (1:10,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG2a secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates GluR1 (~110 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | recombinant monoclonal |
| RH95, monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG2a |
| mol wt | calculated mol wt 101.58 kDa |
| observed mol wt ~110 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P19490  |
| Application: | Anti-GluR1, clone RH95 ZooMAb, Cat. No. ZMS1007, is a recombinant Mouse monoclonal antibody that specifically targets GluR1 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected GluR1 in Mouse Brain tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GluR1 in rat brain tissue sections. Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected GluR1 in rat brain tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GluR1 in rat E18 cortical cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Thyroglobulin-conjugated linear peptide from the N-terminal region of rat Glutamate receptor 1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone RH95 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Glutamate receptor 1 (GluR1). It targets an epitope within the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Glutamate receptor 1 (UniProt: P19490; also known as GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic, AMPA 1, GluA1) is encoded by the Gria1 (also known as Glur1) gene (Gene ID: 50592) in rat. Four different AMPA receptors have been described and are designated as GluR1, GluR2, GluR3, and GluR4. These receptors mediate fast excitatory neurotransmission in the brain and are involved in multiple brain functions, including cognition, movement, learning, and memory. Each of the AMPA receptor subunit can exist in either ′flip′ or ′flop′ form and the efficacy of glutamate is believed to be higher at the ′flip′ form. The binding site on this receptor is formed by the N-terminal tail and the extracellular loop between transmembrane domains three and four. Upon agonist binding, these two loops move towards each other, which opens the pore.Glu-R1 is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains to extracellular domains, four transmembrane domains, and two cytoplasmic domains. It is widely expressed throughout the nervous system and can exist in homotetrameric or heterotetrameric form. Tetramers may be formed by the dimerization of dimers. GluR-1 has several phosphorylation sites on the intracellular carboxy terminus. Many of these sites play a role in receptor regulation and synaptic plasticity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, HK., et al. (2010). J. Physiol. 103(1); 479-489). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |