Anti-MAP2 Antibody, clone AP20 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1013 - recombinant, expressed in HEK 293 cells
Synonym: Microtubule-
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse brain, septal nucleus (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in lateral septal nucleus of mouse brain, septal nucleus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse brain, septal nucleus (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in lateral septal nucleus of mouse brain, septal nucleus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse brain, septal nucleus (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:10 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in lateral septal nucleus of mouse brain, septal nucleus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat hindbrain (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons and nerve processes of rat hindbrain tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat hindbrain (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons and nerve processes of rat hindbrain tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat hindbrain (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons and nerve processes of rat hindbrain tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of E18 cortical neurons was performed using a 1:100 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of E18 cortical neurons was performed using a 1:100 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of E18 cortical neurons was performed using a 1:100 dilution of Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Western Blotting Enhanced Validation-Recombinant Antibody Technology SH-SY5Y cell lysate was probed with Cat. No. ZMS1013, Anti-MAP2, clone AP20 ZooMAb® Mouse Monoclonal (1:1,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG1 secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MAP2 (~260 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AP20, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1 |
| mol wt | calculated mol wt 217 kDa |
| observed mol wt ~260 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, mouse, human |
| species reactivity (predicted by homology) | Xenopus, bovine, quail, chicken |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | F1MEW3  |
| Application: | Anti-MAP2, clone AP20 ZooMAb, Cat. No. ZMS1013, is a recombinant Mouse monoclonal antibody that specifically targets MAP2 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MAP2 in mouse and rat brain tissue lysates. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MAP2 in SH-SY5Y cell line and E18 cortical neurons. Immunohistochemistry (Paraffin) Analysis: A 1:10-100 dilution from a representative lot detected MAP2 in mouse brain and rat brain tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Full-length purified microtubule protein (MAP2) from bovine brain. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone AP20 is a is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects MAP2. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Microtubule-associated protein (UniProt: F1MEW3; also known as MAP2) is encoded by the MAP2 gene (Gene ID: 281294) in bovine. MAP2 is a neuron-specific protein that stabilizes microtubules in the dendrites of postmitotic neurons. Its major function is to stabilize neuronal shape by promoting microtubule synthesis and cross-linking with other components of the cytoskeleton. Phosphorylation of MAP2 controls its association with the cytoskeleton and it is developmentally regulated. MAP2 can undergo phosphorylation by GSK-3 and p44/42 MAP kinase. Phosphorylation of MAP2 at threonine 1620 and 1623 by GSK-3 are reported to inhibits MAP2 association with microtubules and affect microtubule stability. MAP2 consists of at least three isoforms produced from a single gene: high molecular mass MAP2a and MAP2b, and low molecular mass MAP2c that is also known as the juvenile MAP2c, which is expressed only during early development and exists mainly in axons. MAP2a appears to replace MAP2c during growth and MAP2b is expressed throughout life. MAP2 can also serve as a marker of synaptic plasticity and its levels are observed to increase after traumatic brain injury. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Soltani, MH., et al. (2005). Am. J. Pathol. 166(6); 1841-1850). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |