Anti-Cytomegalovirus Antibody, clone 8B1.2 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1015 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) pellet section with MRC-5 cells infected with Cytomegalovirus (A) and Negative control (no primary) (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1015, Anti-Cytomegalovirus, clone 8B1.2 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Nuclear staining was observed in subsets of virus infected MRC-5 cells.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) pellet section with MRC-5 cells infected with Cytomegalovirus (A) and Negative control (no primary) (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1015, Anti-Cytomegalovirus, clone 8B1.2 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Nuclear staining was observed in subsets of virus infected MRC-5 cells.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) pellet section with MRC-5 cells infected with Cytomegalovirus (A) and Negative control (no primary) (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1015, Anti-Cytomegalovirus, clone 8B1.2 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Nuclear staining was observed in subsets of virus infected MRC-5 cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected (A and B) and Cytomegalovirus infected MRC-5 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1015, Anti-Cytomegalovirus, clone 8B1.2 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected (A and B) and Cytomegalovirus infected MRC-5 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1015, Anti-Cytomegalovirus, clone 8B1.2 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected (A and B) and Cytomegalovirus infected MRC-5 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1015, Anti-Cytomegalovirus, clone 8B1.2 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected (A and B) and Cytomegalovirus infected MRC-5 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1015, Anti-Cytomegalovirus, clone 8B1.2 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected (A and B) and Cytomegalovirus infected MRC-5 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1015, Anti-Cytomegalovirus, clone 8B1.2 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
ELISA Enhanced Validation - Recombinant Antibody Technology 60 ng of Cytomegalovirus (CMV) mix antigen was analyzed by ELISA using various dilutions (starting at 200 mg/mL) of Cat. No. ZMS1015, Anti-Cytomegalovirus, clone 8B1.2 ZooMAb® Mouse Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Mouse secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8B1.2, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG2a |
| mol wt | calculated mol wt 62.87 kDa |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| purified by | using protein G |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | virus |
| storage temp. | 2-8°C |
| technique(s) | ELISA: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| UniProt accession no. | P19893  |
| Application: | Quality Control Testing Evaluated by Immunocytochemistry in MRC-5 cells infected with Cytomegalovirus. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected immediate early antigen in MRC-5 cells infected with Cytomegalovirus. Tested applications ELISA Analysis: A representative lot of this antibody detected Cytomegalovirus mix antigens.in ELISA Application. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected immediate early antigen in FFPE cell pellet sections containing cytomegalovirus infected MRC-5 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Cytomegalovirus, clone 8B1.2 ZooMAb®, Cat. No. ZMS1015, is a recombinant Mouse monoclonal antibody that detects immediate early antigen in cytomegalovirus infected cells and is used in ELISA, Immunocytochemistry, and Immunohistochemistry (Paraffin). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | Affinity purified immediate early antigen from MRC-5 cells infected with Human Cytomegalovirus Strain AD169 |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 8B1.2 is a ZooMAb® mouse monoclonal antibody that detects immediate early antigen in cytomegalovirus infected human cells. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Cytomegalovirus (CMV) is a prototype b-herpes virus that is ubiquitously distributed in the human population and is known to cause silent pandemic in immunocompromised subjects. Following a primary infection, which is usually asymptomatic, the virus can establish a latent infection and can be re-activated. The CMV replication cycle has three distinct phases that are designated as immediate early (TE), early (E) and late (L). The immediate early antigens are 72 kDa IE1 and 86 kDa IE2 phosphoproteins that are the first de novo synthesized proteins following primary CMV infection or re-activation. They play an important role in the immune response and are recognized by specific T cells. While IE2 is reported to be absolutely indispensable for productive viral replication, IE1 is only conditionally essential and appears to synergize with IE2 to promote transcriptional activation of the viral early genes. Although IE2 is the principal transcriptional activator of the CMV early genes, it can also negatively regulate viral gene expression after low infection. IE2 is also shown to block virus-induced expression of several inflammatory chemokines, including IL-8, MCP-2, MIG, MIP-1a, and RANTES. It also blocks host cell DNA replication. Both, IE1 and IE2 are reported to delay cell death in infected cells and even block apoptosis. Ectopic IE1 is shown to trigger a p53-dependent G1 growth arrest response, while it induces quiescent cells to enter S phase in p53-negative cells. IE1 also shown to antagonize the type I interferon by interfering with Jak-STAT signaling. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Maidji, E., et al. (2017). PLOS Pathogens. 13(2); e1006202; Christina Paulus, C., and Nevels, M. (2009). Viruses. 1(3); 760-779; Van Zanten, J., et al. (1991). Clin. Exp. Immunol. 83(1); 102-107). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |