Anti-Influenza A Nucleoprotein Antibody, clone A1 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1020 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of MDCK cells infected with Influenza A virus (A & C) or non-infected cells (B & D) was performed using a 1:100 dilution of Cat. No. ZMS1020, Anti-Influenza A Nucleoprotein, clone A1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus, cytoplasm, or both in infected cells
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of MDCK cells infected with Influenza A virus (A & C) or non-infected cells (B & D) was performed using a 1:100 dilution of Cat. No. ZMS1020, Anti-Influenza A Nucleoprotein, clone A1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus, cytoplasm, or both in infected cells
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of MDCK cells infected with Influenza A virus (A & C) or non-infected cells (B & D) was performed using a 1:100 dilution of Cat. No. ZMS1020, Anti-Influenza A Nucleoprotein, clone A1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus, cytoplasm, or both in infected cells
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of MDCK cells infected with Influenza A virus (A & C) or non-infected cells (B & D) was performed using a 1:100 dilution of Cat. No. ZMS1020, Anti-Influenza A Nucleoprotein, clone A1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus, cytoplasm, or both in infected cells
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of MDCK cells infected with Influenza A virus (A & C) or non-infected cells (B & D) was performed using a 1:100 dilution of Cat. No. ZMS1020, Anti-Influenza A Nucleoprotein, clone A1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus, cytoplasm, or both in infected cells
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 mg/mL of non-specific GST protein (Run 1) or recombinant Influenza A virus nucleoprotein (Run 2) were analyzed by affinity binding assay using Cat. No. ZMS1020, Anti-Influenza A Nucleoprotein, clone A1 ZooMAb® Mouse Monoclonal. The binding of this antibody to recombinant Influenza A nucleoprotein displayed a KD of 2.8 x 10-6 whereas binding to non-specific GST protein displayed a KD of 2.2 x 10-3.
ELISA Enhanced Validation - Recombinant Antibody Technology 0.1 mg of recombinant Influenza A Nucleoprotein was analyzed by ELISA using various dilutions (starting at 1 mg/mL) of Cat. No. Anti-Influenza A Nucleoprotein, clone A1 ZooMAb® Mouse Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Mouse secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | A1, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG2a |
| mol wt | calculated mol wt 56.21 kDa |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity (predicted by homology) | influenza A virus |
| storage temp. | 2-8°C |
| technique(s) | ELISA: suitable |
| immunocytochemistry: suitable | |
| UniProt accession no. | P03466  |
| Application: | Quality Control Testing Evaluated by Immunocytochemistry in MDCK cells infected with Influenza A virus. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Influenza A Nucleoprotein in MDCK cells infected with Influenza A virus. Tested applications ELISA Analysis: 1.0 μg/mL from a representative lot detected recombinant Influenza A virus Nucleoprotein. Affinity Binding Assay: A representative lot of this antibody bound recombinant Influenza A Nucleoprotein with a KD of 2.8 x 10-6 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Influenza A Nucleoprotein, clone A1 ZooMAb®, Cat. No. ZMS1020, is a recombinant Mouse monoclonal antibody that detects Influenza A virus nucleoprotein and is tested for use in Affinity Binding Assay, ELISA, and Immunocytochemistry. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Influenza A virus |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone A1 is a ZooMAb® mouse recombinant monoclonal antibody that detects Nucleoprotein N1 of Influenza A virus. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Nucleoprotein (UniProt: P03466; also known as Nucleocapsid protein, Protein N) is encoded by the NP gene (Gene ID: 956531) in Influenza A virus. Influenza A viruses belong to the Orthomyxoviridae family and encapsidates the negative strand viral RNA that protects it from the action of nucleases. Its envelope membrane is reported to carry hemagglutinin, neuraminidase, and the ion channel protein M2. Inside the membrane there are layers of matrix protein 1 and ribonucleoprotein (RNP) complexes that contain viral RNA and binding proteins. RNP serves as template for transcription and replication and must be localized in the host cell nucleus to start an infectious cycle. However, due to its large size, RNP cannot diffuse through the nuclear pore complex and is transported through CRM-1-dependent nuclear export machinery. Viral nucleoprotein contains two nuclear localization signals (NLS) that are responsible for active RNP import into the nucleus. It has been reported that M1 interaction with RNP hides nucleoprotein′s NLS and soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. This dissociation of M1 from RNP unmasks nucleoprotein′s NLS, targeting the RNP to the nucleus. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| Storage Temp. | 2-8°C |