Anti-MAG Antibody, clone 513 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1035 - recombinant, expressed in HEK 293 cells
Synonym: Myelin-associated glycoprotein; Siglec-4a
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in myelinated nerve fibers of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in myelinated nerve fibers of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in myelinated nerve fibers of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. No appreciable staining was observed in human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. No appreciable staining was observed in human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Goat Anti-Mouse IgG and HRP-DAB. No appreciable staining was observed in human cerebral cortex tissue sections.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat hindbrain tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). Membranous staining was observed in fiber tracts of rat hindbrain tissue sections.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat hindbrain tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). Membranous staining was observed in fiber tracts of rat hindbrain tissue sections.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat hindbrain tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). Membranous staining was observed in fiber tracts of rat hindbrain tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Oligodendrocyte precursor cells was performed using a 1:100 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Oligodendrocyte precursor cells was performed using a 1:100 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of Oligodendrocyte precursor cells was performed using a 1:100 dilution of Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Mouse brain microsomal preparation was probed with Cat. No. ZMS1035, Anti-MAG, clone 513 ZooMAb® Mouse Monoclonal (1:1,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG1 secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MAG (~90 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 513, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1 |
| mol wt | calculated mol wt 69.07 kDa |
| observed mol wt ~90 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, mouse |
| species reactivity (predicted by homology) | human, chicken, bovine, frog |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable |
| Application: | Anti-MAG, clone 513 ZooMAb, Cat. No. ZMS1035, s a recombinant Mouse monoclonal antibody that targets Myelin Associated Glycoprotein and is tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected MAG in rat brain tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MAG in rat brain tissue sections. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MAG in Oligodendrocyte precursor cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | L2 epitope-carrying glycoproteins from 1- to 2- day-old chicken brains. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 513 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Myelin-associated glycoprotein. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Myelin-associated glycoprotein (Siglec-4a, MAG) is encoded by the MAG (also known as GMA) gene (Gene ID: 4099) in chicken. MAG is a single-pass type I membrane protein that is expressed in myelin-forming cells when oligodendrites initiate contact with axons. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-516), a transmembrane domain (aa 517-536), and a cytoplasmic domain (aa 518-626). MAG is a member of the immunoglobulin superfamily that is found in both peripheral and CNS myelin, where it plays a role in the formation and maintenance of myelin sheath. It stabilizes contacts between myelinating axons and glial processes. It also participates in the formation of morphologically intact myelin sheath that results in periaxonal cytoplasmic collar of Schwann cells and compact myelin structure of oligodentrocytes. MAG is also considered to be important in maintenance of myelin with aging. MAG protects motoneurons against apoptosis and injury that is mediated via its interaction with neuronal reticulon receptor 4R (RTN4R) and Reticulon-4 receptor-like 2 protein (RTN4RL2). Three isoforms of MAG protein have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Poltorak, M., et al. (1987). J Cell Biol. 105(4); 1893-1899; Quarles, RH. (2007). J. Neurochem. 100(6); 1431-1448). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |