Anti-Rabies glycoprotein Antibody, clone 24-3F-10 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1046 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected L929 (A and B) and Rabies virus infected L929 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1046, Anti-Rabies glycoprotein, clone 24-3F-10 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected L929 (A and B) and Rabies virus infected L929 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1046, Anti-Rabies glycoprotein, clone 24-3F-10 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected L929 (A and B) and Rabies virus infected L929 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1046, Anti-Rabies glycoprotein, clone 24-3F-10 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected L929 (A and B) and Rabies virus infected L929 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1046, Anti-Rabies glycoprotein, clone 24-3F-10 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected L929 (A and B) and Rabies virus infected L929 cells (C and D) was performed using a 1:100 dilution of Cat. No. ZMS1046, Anti-Rabies glycoprotein, clone 24-3F-10 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million Rabies virus infected L929 cells, fixed and permeabilized, was performed using 0.1 μg of Cat. No. ZMS1046, Anti-Rabies glycoprotein, clone 24-3F-10 ZooMAb® Mouse Monoclonal (Yellow histogram) or the equivalent amount of a Mouse IgG2a isotype control (Grey histogram), followed by a PE-conjugated Goat Anti-Mouse IgG2a secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 24-3F-10, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG2a |
| mol wt | calculated mol wt 58.66 kDa |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | QDF46330  |
| purified by | using protein G |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | virus |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| UniProt accession no. | P03524 |
| Application: | Quality Control Testing Evaluated by Immunocytochemistry in L929 cells infected with Rabies virus. Immunocytochemistry Analysis (ICC): A 1:100 dilution of this antibody detected Rabies virus glycoprotein in L929 cells infected with Rabies virus. Tested applications Immunofluorescence Analysis: A representative lot detected Rabies glycoprotein in Immunofluorescence applications (Do-Phong, Johnny., et. al. (2016). Elife. 5:e13214). Flow Cytometry Analysis: 0.1 μg from a representative lot detected Rabies glycoprotein in one million L929 cells infected with Rabies virus. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Rabies glycoprotein, clone 24-3F-10 ZooMAb®, Cat. No. ZMS1046, is a recombinant Mouse monoclonal antibody that detects Rabies virus glycoprotein and is tested for use in Flow Cytometry, Immunocytochemistry, and Immunofluorescence. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | Protein G of Evelyn Rokitniki Abelseth(ERA) strain of Rabies virus. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 24-3F-10 is a ZooMAb® Mouse recombinant monoclonal antibody that detects glycoprotein of Rabies virus. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Glycoprotein (UniProt: P03524) is encoded by the G gene in Rabies virus, strain ERA. Glycoprotein is a single-pass type I membrane glycoprotein that belongs to the lyssavirus glycoprotein family. It attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscle form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Its structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. It is glycosylated and palmitoylated by host and its glycosylation is considered to be crucial for its export at the cell surface. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |