Anti-Fibroblast Antibody, clone TE-7 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1048 - recombinant, expressed in HEK 293 cells
Synonym: Fibrocyte;Fibroblast
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skeletal muscle (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in subsets of myocytes of human skeletal muscle tissue.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skeletal muscle (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in subsets of myocytes of human skeletal muscle tissue.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skeletal muscle (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in subsets of myocytes of human skeletal muscle tissue.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skin (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in papillary dermis of human skin tissue.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skin (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in papillary dermis of human skin tissue.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skin (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in papillary dermis of human skin tissue.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of human foreskin fibroblasts (A and B) and Negative control (No primary antibody) (C and D) were performed using a 1:500 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of human foreskin fibroblasts (A and B) and Negative control (No primary antibody) (C and D) were performed using a 1:500 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of human foreskin fibroblasts (A and B) and Negative control (No primary antibody) (C and D) were performed using a 1:500 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of human foreskin fibroblasts (A and B) and Negative control (No primary antibody) (C and D) were performed using a 1:500 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of human foreskin fibroblasts (A and B) and Negative control (No primary antibody) (C and D) were performed using a 1:500 dilution of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million human foreskin fibroblasts was performed using 0.1 μg of Cat. No. ZMS1048, Anti-Fibroblast, clone TE-7 ZooMAb® Mouse Monoclonal (Yellow histogram) or the equivalent amount of a Mouse IgG1 isotype control (Grey histogram), followed by a PE-conjugated Goat Anti-Mouse IgG1 secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TE-7, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned , Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | Unknown  |
| purified by | using protein G |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| UniProt accession no. | Unknown |
| Application: | Quality Control Testing Evaluated by Immunocytochemistry in human foreskin fibroblasts. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Fibroblast in human foreskin fibroblasts. Tested applications Flow Cytometry Analysis: 0.1 μg from a representative lot detected human foreskin fibroblasts. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibroblast in human skeletal muscle and human skin tissue sections. Immunocytochemistry Analysis: A representative lot detected Fibroblast in Immunocytochemistry applications (Goodpaster, T., et. al. (2008). J Histochem Cytochem. 56(4):347-58). Immunohistochemistry Applications: A representative lot detected Fibroblast in Immunohistohemistry applications (Goodpaster, T., et. al. (2008). J. Histochem. Cytochem. 56(4):347-58). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | Human thymic stroma cells. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone TE-7 is a ZooMAb® Mouse recombinant monoclonal antibody that detects fibroblasts in various human tissues. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Fibroblasts, spindle-shaped cells, are derived from undifferentiated mesenchymal cells and are the most common cells of connective tissue in animals. Their main function is to maintain the structural integrity of connective tissues by continuously secreting precursors of ECM. They are capable of both synthesis and degradation of extracellular matrix (ECM). Unstimulated fibroblasts are biosynthetically quiescent, however, when stimulated by extracellular signals they secrete ECM macromolecules, growth factors, and an array of cytokines. They play a critical role in maintaining homeostasis of the microenvironment and play a critical role in wound healing. During the wound healing process, growth factors released by inflammatory cells stimulate fibroblasts to migrate toward a wound where they proliferate and secrete a collagen-rich extracellular matrix. They differentiate into myofibroblasts and actively close the wound by contraction. Clone TE-7 is a reliable marker for mesodermally derived cells in various fetal and adult tissues and fibrosarcoma. It is also useful for the assessment of thymic epithelial culture contamination with fibroblasts. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goodpaster, T., et al. (2008). J. Histochem. Cytochem. 56(4): 347-358; Haynes, BF., et al. (1984). J. Exp. Med. 159(4); 1149-1168). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |