Anti-Clusterin α chain Antibody, clone 41D ZooMAb® Mouse Monoclonal
SIGMA/ZMS1049 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human adrenal gland (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was primarily observed in medullary cells of human adrenal gland tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human adrenal gland (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was primarily observed in medullary cells of human adrenal gland tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human adrenal gland (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was primarily observed in medullary cells of human adrenal gland tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human spleen (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was primarily observed in medullary cells of human spleen tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human spleen (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was primarily observed in medullary cells of human spleen tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human spleen (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was primarily observed in medullary cells of human spleen tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human tonsil tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the ECM.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human tonsil tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the ECM.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human tonsil tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the ECM.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from HeLa (Lane 1), MCF-7 (Lane 2), and PANC-1 (Lane 3) cells were probed with Cat. No. ZMS1049, Anti-Clusterin α chain, clone 41D ZooMAb® Mouse Monoclonal (1:1,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG1κ secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Clusterin α chain (~60 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 41D, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1κ |
| mol wt | calculated mol wt 52.50 kDa |
| observed mol wt ~60 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_001822.3  |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P10909 |
| Application: | Quality Control Testing Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Clusterin a in HeLa cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Clusterin a chain in MCF-7 and PANC-1 cell lysates. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Clusterin a in human tonsil tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Clusterin a in human adrenal gland and human spleen tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Clusterin chain, clone 41D ZooMAb®, Cat. No. ZMS1049, is a recombinant Mouse monoclonal antibody that detects Clusterin and is tested for use in and Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Full length, purified, native Clusterin from human serum. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 41D is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects human Clusterin a. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Clusterin (UniProt: P10909; also known as Aging-associated gene 4 protein, Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, CLI, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Sulfated glycoprotein 2, SGP-2, Testosterone-repressed prostate message 2, TRPM-2) is encoded by the CLU (also known as APOJ, CLI, KUB1, AAG4) gene (Gene ID: 1191) in human. Clusterin is a ubiquitously expressed protein that functions as extracellular chaperone that prevents aggregation of non-native proteins. It also prevents stress-induced aggregation of plasma proteins. Clusterin is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form that can be proteolytically cleaved into a b-chain (aa 23-227) and an a-chain (aa 228-449). However, proteolytic cleavage is not necessary for its chaperone activity. Six different isoforms of Clusterin have been described that are produced by alternative splicing. Isoform 1 is shown to retro-translocate from the secretory compartments to the cytosol upon cellular stress, but isoforms 4 and 6 retain their cytoplasmic localization in stressed and unstressed cell. It maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70, but does not refold proteins by itself. Clusterin is reported to bind to Amyloid b-peptides and alters their aggregation and promote their clearance, suggesting its neuroprotective role. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Foster, EM., et al. (2019). Front. Neurosci. 13; 64; Rohne, P., et al. (2014). Cell Physiol. Biochem. 34(5); 1626-1639; Amy R Wyatt, AR., and Wilson, MR. (2010). J. Biol. Chem. 285(6); 3532-3539; Poon, S., et al. (2000). Biochemistry. 39(51); 15953-15960). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |