Anti-Dystrophin Antibody, clone 2C6 (MANDYS106) ZooMAb® Mouse Monoclonal
SIGMA/ZMS1051 - recombinant, expressed in HEK 293 cells
Synonym: Anti-BMD; Anti-DXS142; Anti-DXS164; Anti-DXS206; Anti-DXS230; Anti-DXS239; Anti-DXS268; Anti-DXS269; Anti-DXS270; Anti-DXS272; Anti-MRX85
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skeletal muscle (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1051, Anti-Dystrophin, clone 2C6 (MANDYS106) ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in sarcolemma of human skeletal muscle tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skeletal muscle (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1051, Anti-Dystrophin, clone 2C6 (MANDYS106) ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in sarcolemma of human skeletal muscle tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skeletal muscle (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1051, Anti-Dystrophin, clone 2C6 (MANDYS106) ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in sarcolemma of human skeletal muscle tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human skeletal muscle tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1051, Anti-Dystrophin, clone 2C6 (MANDYS106) ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human skeletal muscle tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1051, Anti-Dystrophin, clone 2C6 (MANDYS106) ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human skeletal muscle tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1051, Anti-Dystrophin, clone 2C6 (MANDYS106) ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Human Skeletal Muscle (Left image) and Human Heart (Right image) tissue lysates were probed with Cat. No. ZMS1051, Anti-Dystrophin, clone 2C6 (MANDYS106) ZooMAb® Mouse Monoclonal (1:10,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG2a secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Dystrophin (~427 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | C26, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG2aκ |
| mol wt | calculated mol wt ~426.75 kDa |
| observed mol wt ~425 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| storage temp. | 2-8°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P11532  |
| Application: | Quality Control Testing Evaluated by Western Blotting in Human Skeletal Muscle tissue lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Dystrophin in Human Skeletal Muscle tissue lysate. Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Dystrophin in Human heart tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Dystrophin in human skeletal muscle tissue sections. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Dystrophin in human skeletal muscle tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Dystrophin, clone 2C6 (MANDYS106) ZooMAb®, Cat. No. ZMS1051, is a recombinant Mouse monoclonal antibody that specifically targets Dystrophin and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | TrpE-tagged recombinant fragment corresponding to 110 amino acids from the internal region of human Dystrophin (from the Exon 43-coded pectrin-like repeat 16 region). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone C26 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Dystrophin. It targets an epitope from the internal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Dystrophin (UniProt: P11532; also known as DMD) is encoded by the DMD gene (Gene ID: 1756) in human. Dystrophin is a rod-shaped cytoplasmic protein and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. It is expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Its expression is also reported in brain, muscle, kidney, lung and testis. Dystrophin is localized to the inner part of the muscle fiber cell membrane (sarcolemma), where it forms the dystrophin-associated glycoprotein complex (DGC) that links the extracellular matrix to the actin cytoskeleton. The N-terminus is the actin-binding domain, and the carboxy-terminal domain interacts with beta-dystroglycan as well as dystrobrevin and the syntrophin. The central rod domain that comprises the major mass of the dystrophin molecule forms a flexible, rod-shaped structure. Dystrophin plays an important role in stabilizing the muscle fiber against the mechanical forces of muscle contraction by providing a shock-absorbing connection between the cytoskeleton and the extracellular matrix. The DMD gene is the largest known gene in humans and mutations in this gene are known to cause Duchenne muscular dystrophy, a sex-linked recessive disorder that affects males aged 3 to 7 year as proximal muscle weakness with steady progression. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |