Anti-Integrin αV Antibody, clone 272-17E6 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1053 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in subsets of tubules of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in subsets of tubules of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in subsets of tubules of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Diffuse cytoplasmic staining was observed in trophoblasts and decidual cells of human placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Diffuse cytoplasmic staining was observed in trophoblasts and decidual cells of human placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Diffuse cytoplasmic staining was observed in trophoblasts and decidual cells of human placenta tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cell line was performed using a 1:100 dilution of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cell line was performed using a 1:100 dilution of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cell line was performed using a 1:100 dilution of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million A549 cells was performed using 0.1 μg of Cat. No. ZMS1053, Anti-Integrin αV, clone 272-17E6 ZooMAb® Mouse Monoclonal (Yellow histogram) or the equivalent amount of a Mouse IgG1 isotype control (Grey histogram), followed by a PE-conjugated Goat Anti-Mouse IgG1 secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 272-17E6, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1κ |
| mol wt | calculated mol wt ~116.04 kDa |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| UniProt accession no. | P06756  |
| Application: | Quality Control Testing Evaluated by Immunocytochemistry in A549 cell line. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin aV in A549 cells. Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin V in human kidney and human placenta tissue sections. Flow Cytometry Analysis: 0.1 μg from a representative lot detected Integrin V in one million A549 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Integrin aV, clone 272-17E6 ZooMAb®, Cat. No. ZMS1053, is a recombinant Mouse monoclonal antibody that specifically targets Integrin aV and is tested for use in Flow Cytometry, Immunocytochemistry, and Immunohistochemistry (Paraffin). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | Full-length purified human Integrin aV. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 272-17E6 is a a ZooMAb® mouse recombinant monoclonal antibody that specifically detects human Integrin aV. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Integrin aV (UniProt: P06756; also known as Vitronectin receptor, Vitronectin receptor subunit alpha, CD51) is encoded by the ITGAV (also known as MSK8, VNRA, VNTR) gene (Gene ID: 3685) in human. Integrins are heterodimeric integral membrane proteins composed of an a subunit and a b subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various a and b-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the an a subunit. The structural and functional diversity of the integrin family is based upon the pairing abilities of the individual a and b subunits. Integrins are not constitutively active and their activation from a low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-asparti |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |