Anti-Centrin Antibody, clone 20H5 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1054 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human testis (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1054, Anti-Centrin, clone 20H5 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Cytoskeletal staining was observed in seminiferous tubules and Leydig cells of human testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human testis (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1054, Anti-Centrin, clone 20H5 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Cytoskeletal staining was observed in seminiferous tubules and Leydig cells of human testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human testis (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1054, Anti-Centrin, clone 20H5 ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Cytoskeletal staining was observed in seminiferous tubules and Leydig cells of human testis tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human testis tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1054, Anti-Centrin, clone 20H5 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human testis tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1054, Anti-Centrin, clone 20H5 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human testis tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1054, Anti-Centrin, clone 20H5 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Human (Lane 1) and Mouse (Lane 2) testis tissue lysates were probed with Cat. No. ZMS1054, Anti-Centrin, clone 20H5 ZooMAb® Mouse Monoclonal (1:10,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG2a secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Centrin (~18 kDa).
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12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 20H5, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG2a |
| mol wt | calculated mol wt ~19.46 kDa |
| observed mol wt ~18 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | Chlamydomonas reinhardtii, rat |
| storage temp. | 2-8°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P05434  |
| Application: | Quality Control Testing Evaluated by Western Blotting in Mouse testis tissue lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Centrin in Mouse testis tissue lysate. Tested Applications Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Centrin in human testis tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Centrin in human testis tissue sections. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Centrin in human testis tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Centrin, clone 20H5 ZooMAb®, Cat. No. ZMS1054, is a recombinant Mouse monoclonal antibody that specifically targets Centrin and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | Recombinant Chalamydomonas reinhardtii centrin. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 20H5 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects centrin (Caltractin). It targets an epitope within the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Caltractin (UniProt: P05434; also known as 20 kDa calcium-binding protein, Centrin) is encoded by the VFL2 gene (Gene ID: 5725094) in Chlamydomonas reinhardtii (algae). Centrin is a highly conserved calmodulin-like calcium-binding phospho-protein that contain four EF hands and are associated with centrosome-related structures. In Chlamydomonas reinhardtii, centrin is shown to play an essential role in microtubule severing during flagellar excision during which time stellate fibers of the transition zone contract and displace the microtubule doublets of the axoneme inward. The resulting shear force and torsional load generated during inward displacement leads to microtubule severing immediately distal to the central cylinder of the transition zone. Centrin is also present is all eukaryotic cells and contain four helix-loop-helix motifs that all conform to the EF-hand consensus for Ca2+ binding. Two of these motifs exhibit high affinity for Ca2+ two display lower affinity. Stability of centrin is regulated by phosphorylation of serine residue in the C-terminal region by aurora kinase A, which also localizes with centrin at the centrosome. Their levels are shown to be the highest from prophase to metaphase stage followed by precipitous loss in the late stage of mitosis. In human breast cancers higher levels of aurora kinase A and phospho-centrin have been observed. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sanders, MA., and Salisbury, JL. (1994). J. Cell Biol. 24(5); 795-805; Lutz, W., et al. (2001). J. Biol. Chem. 276(23); 20774-20780; Lukasiewicz, KB., et al. (2011). PLoS ONE 6(6); e21291). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |