Anti-RSV Fusion Protein Antibody, clone 131-2A ZooMAb® Mouse Monoclonal
SIGMA/ZMS1055 - recombinant, expressed in HEK 293 cells
Synonym: Fusion Glycoprotein
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) RSV infected HEp-2 cells (A) and Negative control (no primary) (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in subsets of RSV-infected HEp-2 cells.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) RSV infected HEp-2 cells (A) and Negative control (no primary) (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in subsets of RSV-infected HEp-2 cells.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) RSV infected HEp-2 cells (A) and Negative control (no primary) (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal. Reactivity was detected using a Rabbit Anti-Mouse IgG and HRP-DAB. Membranous staining was observed in subsets of RSV-infected HEp-2 cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected HEp-2 cells (A and B) and RSV infected HEp-2 cells (C and D) was performed using a 1:1,000 dilution of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell surface and cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected HEp-2 cells (A and B) and RSV infected HEp-2 cells (C and D) was performed using a 1:1,000 dilution of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell surface and cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected HEp-2 cells (A and B) and RSV infected HEp-2 cells (C and D) was performed using a 1:1,000 dilution of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell surface and cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected HEp-2 cells (A and B) and RSV infected HEp-2 cells (C and D) was performed using a 1:1,000 dilution of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell surface and cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of uninfected HEp-2 cells (A and B) and RSV infected HEp-2 cells (C and D) was performed using a 1:1,000 dilution of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell surface and cytoplasm.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from uninfected HEp-2 cells (Lane 1) and RSV infected HEp-2 cells (Lane 2) were probed with Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Mouse IgG2a secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RSV Fusion Protein (~45 kDa).
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million RSV infected HEp-2 cells was performed using 1 μg of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal (Yellow histogram) or the equivalent amount of a Mouse IgG2a isotype control (Grey histogram), followed by a PE-conjugated Goat Anti-Mouse IgG2a secondary antibody.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million RSV infected HEp-2 cells was performed using 1 μg of Cat. No. ZMS1055, Anti-RSV Fusion Protein, clone 131-2A ZooMAb® Mouse Monoclonal (Orange histogram) or uninfected HEp-2 cells (Grey histogram), followed by a PE-conjugated Goat Anti-Mouse IgG2a secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 131-2A, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG2aκ |
| mol wt | calculated mol wt 63.45 kDa |
| observed mol wt ~45 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | QEI22738.1  |
| purified by | using protein G |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | virus |
| storage temp. | 2-8°C |
| technique(s) | ELISA: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P03420 |
| Application: | Quality Control Testing Evaluated by Immunocytochemistry in RSV infected HEp-2 cells. Immunocytochemistry Analysis (ICC): A 1:1,000 dilution of this antibody detected RSV Fusion Protein in RSV infected HEp-2 cells. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RSV Fusion Protein in lysate from RSV infected HEp-2 cells. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected RSV Fusion Protein in RSV infected HEp-2 cells. ELISA Analysis: A representative lot detected RSV Fusion Protein in ELISA applications (Newby, J., et. al. (2017). Nat Commun. 8(1):833). Flow Cytometry Analysis: 1 μg from a representative lot detected RSV Fusion Protein in one million RSV infected HEp-2 cells. Flow Cytometry Analysis: A representative lot detected RSV Fusion Protein in Flow Cytometry applications (Preugschas, H.F., et. al. (2019). Cell Microbiol. 21(1):e12955). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | A2 Respiratory Syncytial Virus (RSV) extract. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 131-2A is a Mouse recombinant monoclonal antibody that detects fusion protein in Respiratory syncytial virus (RSV). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Fusion glycoprotein F0 (UniProt: P03420) is encoded by the F gene (Gene ID: 1494475) in Human respiratory syncytial virus A (strain A2). Human respiratory syncytial virus (hRSV) is a pneumovirus that causes significant respiratory disease in premature and full-term infants. Fusion glycoprotein F0 of RSV is synthesized with a signal peptide (aa 1-15), which is subsequently cleaved off. The inactive F0 precursor is then cleaved at two sites (aa 109-110 and 136-137) by furin-like protease to give rise to produce mature F1 and F2 fusion glycoproteins. Fusion glycoprotein F1 is a single-pass type I membrane protein that can exist as homotrimer or can form disulfide-linked heterodimer with fusion glycoprotein F2. Its N-terminus has a hydrophobic fusion peptide that inserts into the target host membrane. It is buried in the center of the trimer cavity before cleavage by host furin. The fusion glycoprotein F2 is the major determinant of the species specificity of RSV infection. Fusion protein can exist in at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the coiled coil regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. This fusion is pH independent and occurs at the plasma or endosomal membrane. The fusion protein is reported to be involved in the entry into the host cell through the interaction with host IGF-R1. This interaction activates protein kinase z that recruits host NCL/nucleolin to the apical cell surface where it can bind fusion glycoprotein F1. Later in infection, fusion protein expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. Fusion protein can also trigger p53-dependent apoptosis in cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: McLellan, JS., et al. (2013). Science. 340(6136); 1113-1117; Olivier, A., et al. (2009). Int. J. Exp. Pathol. 90(4); 431-438; Schlender, J., et al. (2003). J. Virol. 77(8); 4609-4616). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |