Anti-Cytokeratin 5/6 Antibody, clone D5/16 B4 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1057 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in non-germinal center of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in non-germinal center of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in non-germinal center of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skin (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in epidermis of human skin tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skin (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in epidermis of human skin tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human skin (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in epidermis of human skin tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human skin tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton, nucleus.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human skin tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton, nucleus.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of human skin tissue sections was performed using a 1:100 dilution of Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton, nucleus.
Western Blotting Enhanced Validation - Recombinant Antibody Technology A431 cell lysate was probed with Cat. No. ZMS1057, Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb® Mouse Monoclonal (1:1,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG1 secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrows indicate Cytokeratin 5 and 6 (~55-62 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
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Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | D5/16 B4, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1 |
| mol wt | calculated mol wt 62.38 kDa |
| observed mol wt ~62 and 55 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_000415.2  |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P13647 |
| Application: | Quality Control Testing Evaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Cytokeratin 5/6 in human tonsil tissue sections. Tested applications Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cytokeratin 5/6 in human skin tissue sections. Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cytokeratin 5/6 in A431 and HaCaT cell lysates. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Cytokeratin 5/6 in human skin tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb®, Cat. No. ZMS1057, is a Mouse recombinant antibody that detects Cytokeratin 5/6 and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Purified human Cytokeratin 5. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone D5/16 B4 is a a ZooMAb® mouse recombinant monoclonal antibody that detects human cytokeratins 5 and 6. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Keratin, type II cytoskeletal 5 (UniProt: P13647; also known as 58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5) is encoded by the KRT5 gene (Gene ID: 3852) in human. Keratins are the intermediate filament proteins of epithelia that display high degree of molecular diversity. They are heteropolymeric filaments formed by pairing of type I and type II keratins that are expressed in a highly specific patterns depending on the epithelial type and stage of cellular differentiation. Keratins contain a central rod domain of about 310 amino acids with alpha-helical conformation bordered by non-helical head and tail domains of variable length. The head domain consists of subdomains V1 and H1. The central alpha helical rod domain is composed of subdomains 1A, 1B, 2A, and 2B connected by the linkers L1, L12, and L2. The tail domain then consists of subdomains H2 and V2. They provide stability between epithelial cells and their attachment to basement membrane. Keratins also serve as tumor markers and epithelial differentiation markers. Multiple human epithelial keratins have been resolved with 2D-gels electrophoresis. These can be divided into acidic (pI |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |