Anti-MMP14 Antibody, clone LEM-2/15.8 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1060 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1060, Anti-MMP14, clone LEM-2/15.8 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in glandular cells, lamina propria & submucosa of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1060, Anti-MMP14, clone LEM-2/15.8 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in glandular cells, lamina propria & submucosa of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZMS1060, Anti-MMP14, clone LEM-2/15.8 ZooMAb® Mouse Monoclonal. Reactivity was detected using an Rabbit Anti-Mouse IgG and HRP-DAB. Cytoplasmic/membranous/ECM staining was observed in glandular cells, lamina propria & submucosa of human colon tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZMS1060, Anti-MMP14, clone LEM-2/15.8 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZMS1060, Anti-MMP14, clone LEM-2/15.8 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZMS1060, Anti-MMP14, clone LEM-2/15.8 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane.
Western Blotting Enhanced Validation - Recombinant Antibody Technology HepG2 cell lysate was probed with Cat. No. ZMS1060, Anti-MMP14, clone LEM-2/15.8 ZooMAb® Mouse Monoclonal (1:1,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG1 secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MMP14 (~55 kDa).
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million HepG2 cells was performed using 1 μg of Cat. No. ZMS1060, Anti-MMP14, clone LEM-2/15.8 ZooMAb® Mouse Monoclonal (Yellow histogram) or the equivalent amount of a Mouse IgG1 isotype control (Grey histogram), followed by a PE-conjugated Goat Anti-Mouse IgG1 secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LEM-2/15.8, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Extracellular domain |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1 |
| mol wt | calculated mol wt 65.89 kDa |
| observed mol wt ~55 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | rat, monkey, mouse, bovine |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P50281  |
| Application: | Quality Control Testing Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MMP14 in HepG2 cell lysate. Tested applications Flow Cytometry Analysis: 1 μg from a representative lot detected MMP14 in one million HepG2 cells. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MMP14 in human colon tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MMP14 in HepG2 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-MMP14, clone LEM-2/15.8 ZooMAb®, Cat. No. ZMS1060, is a recombinant Mouse monoclonal antibody that targets MMP14 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Linear Peptide corresponding to 16 amino acids from the extracellular domain of human Matrix Metalloproteinase-14 (MMP14). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone LEM-2/15.8 is a mouse recombinant monoclonal antibody that specifically detects Matrix metalloproteinase-14 (MMP14). It targets an epitope within 16 amino acids from the extracellular domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Matrix metalloproteinase-14 (UniProt: P50281; also known as EC:3.4.24.80, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP) is encoded by the MMP14 gene (Gene ID: 4323) in human. MMPs are a family zinc-dependent endoproteinases that degrade collagen and other structural proteins and are responsible for much of the turnover of matrix components. Each MMP contains a protease domain with a conserved HExGHxxGxxHS/T sequence, where three histidine residues form a complex with a catalytic Zinc atom. A regulatory domain with a conserved PRCGxPD motif helps to maintain MMPs in their latent form by binding of the cysteine residue to the active site Zn. MMP-14 is a single-pass type I membrane protein that is expressed in stromal cells of colon, breast, head, and neck. It is a membrane type matrix metalloproteinase (MT-MMP) that is synthesized with a signal peptide (aa 1-20) and a propeptide (aa 21-111) that are subsequently cleaved off to generate the active mature form. The propeptide maintains it in a latent state. The mature enzyme contains an extracellular domain (aa 112-541), a transmembrane domain (aa 542-562), and a cytoplasmic domain (aa 563-582). MMP14 contains four hemopexin domains within its extracellular domain and a cysteine switch (aa 91-98). The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the release of propeptide activates the enzyme. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |