Anti-Dynein Antibody, clone 74.1 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1064 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluroresent analysis of HeLa cells was performed using a 1:100 dilution of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluroresent analysis of HeLa cells was performed using a 1:100 dilution of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluroresent analysis of HeLa cells was performed using a 1:100 dilution of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluroresent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluroresent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluroresent analysis of HepG2 cells was performed using a 1:100 dilution of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluroresent analysis of NIH3T3 cells was performed using a 1:100 dilution of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluroresent analysis of NIH3T3 cells was performed using a 1:100 dilution of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluroresent analysis of NIH3T3 cells was performed using a 1:100 dilution of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from A431 (Lane 1), HEK293 (Lane 2), and PC12 (Lane 3) cells were probed with Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal (1:1,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG2b secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Dynein (~78 kDa).
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million HeLa cells was performed using 0.1 μg of Cat. No. ZMS1064, Anti-Dynein, clone 74.1 ZooMAb® Mouse Monoclonal (Yellow histogram) or the equivalent amount of a Mouse IgG2b isotype control (Grey histogram), followed by a PE-conjugated Goat Anti-MouseIgG2b secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 74.1, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG2b |
| mol wt | calculated mol wt ~68.56 kDa |
| observed mol wt ~78 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human, mouse |
| species reactivity (predicted by homology) | Xenopus, bovine, sheep, fish, Drosophila |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q29RQ3  |
| Application: | Quality Control Testing Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Dynein in HEK293 cell lysate. Tested Applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Dynein in A431 and PC12 cell lysate. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Dynein in HeLa, HepG2, and NIH 3T3 cells. Flow Cytometry Analysis: 0.1 μg from a representative lot detected Dynein in one million HeLa cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Dynein, clone 74.1 ZooMAb®, Cat. No. ZMS1064, is a recombinant Mouse monoclonal antibody that specifically targets Dynein and is tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | Purified native bovine brain cytoplasmic dynein. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 74.1 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects cytoplasmic dynein. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Cytoplasmic dynein 1 intermediate chain 1 (UniProt: Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) is encoded by the DYNC1I1 gene (Gene ID: 613724) in bovine. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 It is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. It is a multi-subunit complex of 1.5 MDa composed of a homodimer of heavy chains, two intermediate chains, light-intermediate chains, and light chains. The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. Cytoplasmic dynein 1 intermediate chain 1 acts as one of the several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are involved in linking dynein to cargos and to adapter proteins that regulate dynein function. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |