Anti-LINE-1 ORF1p Antibody, clone 4H1 ZooMAb® Mouse Monoclonal
SIGMA/ZMS1066 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of MCF-7 cells was performed using a 1:1,000 dilution of Cat. No. ZMS1066, Anti-LINE-1 ORF1p, clone 4H1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of MCF-7 cells was performed using a 1:1,000 dilution of Cat. No. ZMS1066, Anti-LINE-1 ORF1p, clone 4H1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of MCF-7 cells was performed using a 1:1,000 dilution of Cat. No. ZMS1066, Anti-LINE-1 ORF1p, clone 4H1 ZooMAb® Mouse Monoclonal and visualized with a Goat Anti-Mouse secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation - Recombinant Antibody Technology MCF-7 (Lane 1) and NTERA-2 (Lane 2) cell lysates were probed with Cat. No. ZMS1066, Anti-LINE-1 ORF1p, clone 4H1 ZooMAb® Mouse Monoclonal (1:1,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG1 secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates LINE-1 ORF1p (~40 kDa).
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million MCF-7 cells was performed using 0.1 μg of Cat. No. ZMS1066, Anti-LINE-1 ORF1p, clone 4H1 ZooMAb® Mouse Monoclonal (Yellow histogram) or the equivalent amount of Mouse IgG1 isotype control (Grey histogram), followed by a PE-conjugated Goat Anti-MouseIgG1 secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4H1, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG1κ |
| mol wt | calculated mol wt ~40.06 kDa |
| observed mol wt ~40 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9UN81  |
| Application: | Quality Control Testing Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LINE-1 ORF1p in MCF-7 cell lysate. Tested Applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LINE-1 ORF1p in NTERA-2 cell lysate. Flow Cytometry Analysis: 0.1 μg from a representative lot detected LINE-1 ORF1p in one million MCF-7 cells. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected LINE-1 ORF1p in MCF-7 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-LINE-1 ORF1p, clone 4H1 ZooMAb®, Cat. No. ZMS1066, is a recombinant Mouse monoclonal antibody that specifically targets LINE-1 ORF1p and is tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | A linear peptide corresponding to 10 amino acids from the N-terminal region of human LINE-1 ORF1 protein. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4H1 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects LINE-1 retrotransposable element ORF1 protein. It targets an epitope within 10 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | LINE-1 retrotransposable element ORF1 protein (UniProt: Q9UN81; also known L1ORF1p, LINE retrotransposable element 1, LINE1 retrotransposable element 1) is encoded by the LIRE1 (also known as LRE1) gene in human. LINE1-ORF 1P is a homotrimeric protein of the transposase 22 family that can also form larger homooligomers. It is mainly a cytoplasmic protein and is enriched in stress granules, but may also be detected in the nucleolus. It contains a coiled coil domain that mediates its homotrimerization. Its RNA recognition motif (RRM) (aa 157-252) in the C-terminal domain are required for its proper RNA-binding activity. LINE1-ORF 1P serves as a nucleic acid-binding protein that is essential for retrotransposition of LINE-1 elements in the genome. It is also reported to function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded. Retrotransposition is a shown to be a complex process that involves transcription of the LINE1, transport of its RNA to the cytoplasm, translation of the bicistronic RNA, formation of a ribonucleoprotein particle, its re-import to the nucleus and then target-primed reverse transcription at the integration site. LINE1-ORF 1P expression is consistently detected in p53 mutant tumors, but, little or no expression is detected in Wilms tumors that were normal for p53. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goodier, JL et al. (2013). Nucleic Acids Res. 41(15): 7401-7419). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |