Anti-beta Catenin Antibody, clone 3J7, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB05665 - recombinant, expressed in HEK 293 cells
Synonym: Catenin beta-1
Product Type: Chemical
antibody form | purified antibody |
antibody product type | primary antibodies |
biological source | rabbit (recombinant) |
clone | 3J7, monoclonal |
recombinant monoclonal | |
conjugate | unconjugated |
enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation ![]() |
form | lyophilized |
greener alternative category | Aligned ![]() |
greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry ![]() |
isotype | IgG |
mol wt | calculated mol wt 85.5 kDa |
observed mol wt ~85 kDa | |
packaging | antibody small pack of 25 μL |
product line | ZooMAb® learn more ![]() |
recombinant | expressed in HEK 293 cells |
shipped in | ambient |
species reactivity | human, mouse |
species reactivity (predicted by homology) | rat |
storage temp. | 2-8°C |
technique(s) | immunocytochemistry: suitable using 1:100 |
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100 | |
western blot: suitable using 1:1,000 | |
UniProt accession no. | P35222 ![]() ![]() |
Application: | Anti-beta Catenin, clone 3J7 ZooMAb, Cat. No. ZRB05665, is a recombinant Rabbit Monoclonal Antibody that detects beta-catenin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected beta-Catenin in NIH/3T3 cell lysate. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected beta-Catenin in HUVEC and A431 cells. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected beta- Catenin in human pancreas tissue sections. |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here ![]() |
General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. ![]() |
Immunogen: | GST/His-tagged recombinant fragment corresponding to the first 100 amino acids from the N-terminal region of human beta-catenin. |
Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
Reconstitution: | 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
Specificity: | Clone 3J7 is a ZooMAb rabbit recombinant monoclonal antibody that detects beta-catenin. It targets an epitope with in the N-terminal region of isoform 1. |
Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
Target description: | Catenin beta-1 (UniProt: P35222; also known as beta-catenin) is encoded by the CTNNB1 (also known as CTNNB, OK/SW-cl.35, PRO2286) gene (Gene ID: 1499) in human. Catenin beta-1 is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3 beta that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decrease its nuclear localization and transcriptional activity. Wnt/beta-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC beta-catenin is trimethylated on Lysine 49 by Ezh2 methyltransferase and this trimethylated form represses neuronal differentiation genes Sox1 and Sox3. Mutations in CTNNB1 gene are shown to cause colorectal cancers, medulloblastoma, and ovarian cancers. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hoffmeyer, K., et al. (2017). Cell Reports 18(12); 2815-2824). |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 12352203 |