Anti-DNMT1 Antibody, clone 1L14 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1680 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
antibody form | purified antibody |
antibody product type | primary antibodies |
biological source | rabbit |
clone | 1L14, recombinant monoclonal |
conjugate | unconjugated |
description | recombinant, expressed in HEK 293 cells |
enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
epitope sequence | C-terminal |
form | lyophilized |
greener alternative category | Aligned |
greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
isotype | IgG |
mol wt | calculated mol wt 183.16 kDa |
observed mol wt ~180 kDa | |
packaging | antibody small pack of 25 μL |
product line | ZooMAb® learn more |
Protein ID accession no. | NP_001370 |
purified by | using Protein A |
Quality Level | 200 |
recombinant | expressed in HEK 293 cells |
shipped in | ambient |
species reactivity | human |
species reactivity (predicted by homology) | rat, canine, monkey, bovine, feline, mouse |
storage temp. | 2-8°C |
technique(s) | affinity binding assay: suitable |
immunocytochemistry: suitable | |
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
western blot: suitable | |
UniProt accession no. | P26358 |
Application: | Quality Control Testing Evaluated by Western Blotting in K562 cell nuclear extract. Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected DNMT1 in K562 cell nuclear extract. Tested applications Western Blotting Analysis: A 1:1,000 dilution from representative lot detected DNMT1 in HeLa and NTERA-2 cell nuclear extracts and recombinant human DNMT1. Immunocytochemistry Analysis: A 1:100 dilution from representative lot detected DNMT1 in HeLa cells. Affinity Binding Assay: A representative lot of this antibody bound DNMT1 with a KD of 1.1 x 10-7 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from representative lot detected DNMT1 in human tonsil tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
Immunogen: | KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of human DNA (cytosine-5)-methyltransf |
Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
Specificity: | Clone 1L14 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects DNA (cytosine-5)-methyltransf |
Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
Target description: | DNA (cytosine-5)-methyltransf |
Storage Temp. | 2-8°C |
UNSPSC | 12352203 |