Anti-ASS1 Antibody, clone 2E22 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1817 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1817, Anti-ASS1, clone 2E22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic staining was observed in tubules of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1817, Anti-ASS1, clone 2E22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic staining was observed in tubules of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1817, Anti-ASS1, clone 2E22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit and HRP-DAB. Cytoplasmic staining was observed in tubules of human kidney tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1817, Anti-ASS1, clone 2E22 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Cytoplasmic.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1817, Anti-ASS1, clone 2E22 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Cytoplasmic.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1817, Anti-ASS1, clone 2E22 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Cytoplasmic.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from Raji (Lane 1), HepG2 (Lane 2) cells were probed with Cat. No. ZRB1817, Anti-ASS1, clone 2E22 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ASS1 (~46 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100ug/ml of non-specific control peptide (Run 1) or ASS1 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1817, Anti-ASS1, clone 2E22 ZooMAb® Rabbit Monoclonal. The binding of this antibody to ASS1 peptide displayed a KD of 1.3 x 10^-7 whereas binding to non-specific control peptide displayed a KD of 4.0 x 10^-3.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million Jurkat cells was performed using 0.1 μg of Cat. No. ZRB1817, Anti-ASS1, clone 2E22 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2E22, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Internal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 46.53 kDa |
| observed mol wt ~46 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_000041  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | porcine, chimpanzee, bovine |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P00966 |
| Application: | Quality Control Testing Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Arginosuccinate Synthase (ASS1) in HepG2 cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ASS1 in Raji cell lysates. Affinity Binding Assay: A representative lot of this antibody bound ASS1 with a KD of 1.3 x 10-7 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ASS1 in NIH3T3 cells. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ASS1 in human kidney tissue sections. Flow Cytometry Analysis: 0.1 μg from a representative lot detected ASS1 in one million Jurkat cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 24 amino acids from the internal region of human Arginosuccinate synthase (ASS1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2E22 is a C13 Arginosuccinate synthase (ASS1). It targets an epitope within 24 amino acids from the internal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Arginosuccinate synthase (UniProt: P00966; also known as EC:6.3.4.5, Citrulline--aspartate ligase) is encoded by the ASS1 gene (Gene ID: 445) in human. Arginosuccinate synthase (ASS) is a cytosolic enzyme with highest expression in the liver, renal proximal tubules, and testis. In liver, it is distributed homogeneously and is not confined to periportal cells. In liver, argininosuccinate synthase is reported to associate with the outside of the mitochondria to complete the urea cycle, which involves both the mitochondrial and cytosolic compartments. ASS is a rate-limiting enzyme in the urea cycle. It catalyzes the formation of arginosuccinate from aspartate, citrulline, and ATP. ASS is shown to participate in five major pathways including urea synthesis, nitric oxide synthesis, polyamine synthesis, creatine synthesis, and de novo synthesis of arginine to maintain serum levels. Mutations in ASS1 gene has been linked to citrullinemia type I deficiency that is characterized primarily by elevated serum and urine citrulline levels and ammonia intoxication that is usually manifested in the first few days of life. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Haines, RJ., et al. (2011). Int. J. Biochem. Mol. Biol. 2(1); 8-23; Goodwin, BL., et al. (2004). J. Biol. Chem. 279(18); 18353-18360). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |