Anti-IQGAP1 Antibody, clone 4M20 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1820 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1820, Anti-IQGAP1, clone 4M20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in glandular cells & lamina propria of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1820, Anti-IQGAP1, clone 4M20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in glandular cells & lamina propria of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1820, Anti-IQGAP1, clone 4M20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in glandular cells & lamina propria of human colon tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from HEK293 (Lane 1), CHO K1 (Lane 2), COS-7 (Lane 3), and A549 (Lane 4) cells were probed with Cat. No. ZRB1820, Anti-IQGAP1, clone 4M20 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates IQGAP1 (~190 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or IQGAP1 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1820, Anti-IQGAP1, clone 4M20 ZooMAb® Rabbit Monoclonal. The binding of this antibody to IQGAP1 peptide displayed a KD of 5.6 x 10-7 whereas binding to non-specific control peptide displayed a KD of 1.2 x 10-2.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 4M20, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 189.25 kDa |
| observed mol wt ~190 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_003861  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | hamster, monkey, human |
| species reactivity (predicted by homology) | mouse, porcine, rat |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P46940 |
| Application: | Quality Control Testing Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IQGAP1 in HEK293 cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IQGAP1 in CHO K1, COS-7, and A549 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected IQGAP1 in human colon tissue sections. Affinity Binding Assay: A representative lot of this antibody bound IQGAP1 with a KD of 5.6 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Ras GTPase-activating-like protein IQGAP1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4M20 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Ras GTPase-activating-like protein IQGAP1. It targets an epitope within 18 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Ras GTPase-activating-like protein IQGAP1 (UniProt: P46940; also known as p195) is encoded by the IQGAP1 (also known as KIAA0051) gene (Gene ID: 8826) in human. IQGAP1 is a cell-cell contact regulator that also plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Its expression is reported in the placenta, lung, and kidney and low level of expression is observed in the heart, liver, skeletal muscle, and pancreas. In eukaryotic cells IQGAP1 binds to and alters the function of several proteins, including actin, E-cadherin, beta-catenin, Cdc42, and Rac1. It is localized in the nucleus, cytoplasm, and membrane and its subcellular distribution is regulated by the cell cycle. Its nuclear levels are reported to increase at the G1/S phase. It may also play a role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest. IQGAP1 contains a calponin homology domain (aa 44-159 that is responsible for actin binding, a WW motif (aa 679-712) that is essential for the association of ERK2, four calmodulin-binding IQ domains (aa 745-864), and a RasGAP-related domain (aa 1004-1237) that binds the small GTPases Cdc42 and Rac1. IQGAP1 is reported to inhibit GTPase activity of Cdc42 and overexpression of IQGAP1 in cells is shown to increase Cdc42-GTP levels. Two short C-terminal fragments of IQGAP1 (C1: aa 956-1274; and C2: 1276-1657) are shown to bind exclusively to nucleotide-depleted Cdc42 (Cdc42-ND), whereas the entire C-terminus (aa 956-1657) binds predominantly to the Cdc42-GTP S. Phosphorylation of IQGAP1 at Ser 1443 by protein kinase Cϵ is reported to inhibit the intramolecular interaction of the C-terminal fragments. Ser 1443 phosphorylation causes an opening of the IQGAP1 structure and exposes C1 and C2 regions. Ser 1443 phosphorylation also enhances the ability to promote neurite outgrowth. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, Z., et al. (2005). J. Biol. Chem. 280(14);13871-13878; Grohmanova, K., et al. (2004). J. Biol. Chem. 279(47); 48495-48504). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |