Anti-PDI Antibody, clone 1M19 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1846 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1846, Anti-PDI, clone 1M19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in glandular cells & lamina propria of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1846, Anti-PDI, clone 1M19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in glandular cells & lamina propria of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1846, Anti-PDI, clone 1M19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in glandular cells & lamina propria of human colon tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cells was performed using a 1:100 dilution of Catalog no. ZRB1846, Anti-PDI, clone 1M19 ZooMAb® Rabbit Monoclonal and visualised with a Goat, Anti-Rabbit secondary antibody conjugated to Alexa fluor® 488 (Green). Actin filaments have been labelled with phalliodin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm/ endoplasmic reticulum of the membrane.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cells was performed using a 1:100 dilution of Catalog no. ZRB1846, Anti-PDI, clone 1M19 ZooMAb® Rabbit Monoclonal and visualised with a Goat, Anti-Rabbit secondary antibody conjugated to Alexa fluor® 488 (Green). Actin filaments have been labelled with phalliodin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm/ endoplasmic reticulum of the membrane.
Immunocytochemistry Enhanced
Validation - Recombinant Antibody Technology Immunofluorescent
analysis of NIH3T3 cells was performed using a 1:100 dilution of Catalog no.
ZRB1846, Anti-PDI, clone 1M19 ZooMAb® Rabbit Monoclonal and
visualised with a Goat, Anti-Rabbit secondary antibody conjugated to Alexa fluor®
488 (Green). Actin filaments have been labelled with phalliodin (Red). Nucleus
is stained with DAPI (Blue). This antibody positively stains the cytoplasm/
endoplasmic reticulum of the membrane.
Validation - Recombinant Antibody Technology Immunofluorescent
analysis of NIH3T3 cells was performed using a 1:100 dilution of Catalog no.
ZRB1846, Anti-PDI, clone 1M19 ZooMAb® Rabbit Monoclonal and
visualised with a Goat, Anti-Rabbit secondary antibody conjugated to Alexa fluor®
488 (Green). Actin filaments have been labelled with phalliodin (Red). Nucleus
is stained with DAPI (Blue). This antibody positively stains the cytoplasm/
endoplasmic reticulum of the membrane.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from HeLa (Lane 1), A431 (Lane 2), NIH3T3 (Lane 3), and PC12 (Lane 4) cells were probed with Cat. No. ZRB1846, Anti-PDI, clone 1M19 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PDI (Protein Disulfide Isomerase) (~58 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of non-specific control peptide (Run 1) or Protein Disulfide Isomerase (PDI) peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1846, Anti-PDI, clone 1M19 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Protein Disulfide Isomerase peptide displayed a KD of 3.2 x 10-9 whereas binding to non-specific control peptide displayed a KD of 2.2 x 10-4.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1M19, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 57.12 kDa |
| observed mol wt ~58 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_000909  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human, mouse |
| species reactivity (predicted by homology) | monkey, porcine, bovine, feline, canine |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P07237 |
| Application: | Quality Control Testing Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PDI in A431 cell lysate. Tested applications Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected PDI in HeLa, NIH3T3, and PC12 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PDI in human colon tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PDI in NIH3T3 cells. Affinity Binding Assay: A representative lot of this antibody bound Protein Disulfide Isomerase (PDI) peptide with a KD of 3.2 x 10-9 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Protein disulfide-isomerase (PDI). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone IM19 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Protein disulfide-isomerase (PDI). It targets an epitope within 14 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Protein disulfide-isomerase (UniProt: P07237; also known as EC:5.3.4.1, PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55) is encoded by the P4HB (also known as ERBA2L, PDI, PDIA1, PO4DB) gene (Gene ID: 5034) in human. PDI is a multifunctional redox chaperone of the endoplasmic reticulum (ER). It is synthesized with a signal peptide (aa 1-17) that is subsequently cleaved off to produce the mature form. It is abundantly expressed in various tissues and may account for up to 0.8% of the total cellular protein. It is induced during ER stress and serves as a critical cellular defense against protein misfolding. It can distinguish between partially folded, unfolded, and properly folded proteins, and it has a higher affinity to bind to misfolded proteins. It is also involved in isomerization, formation, and rearrangement of protein disulfide bonds to ensure maintenance of protein conformation. At high concentrations it is reported to function as a chaperone that inhibits aggregation of misfolded proteins, however; at low concentrations it facilitates aggregation. PDI consists of four domains known as a, b, b′, a′. The homologous a and a domains contain the active site, CGHC. The individual a and a′ domains have similar oxidizing ability but have low isomerase activity. The b′ domain is the main site for binding misfolded protein substrates but the other domains also contribute to this process. PDI also contains two Thioredoxin domains (aa 18-134 and 349-475). The CGHC motif modulates the overall reduction potential of PDI and thus it regulates the catalytic ability of the active site cysteines to actively oxidize or reduce disulfide bonds. PDI also contains a x linker region and an acidic C-terminal (aa 476-508) containing a KDEL-ER retrieval sequence. Deletion of the C-terminal residues of PDI is shown to result in deactivation of its chaperone-like activity and its peptide binding ability, however, it does not affect its catalytic activity in disulfide bond formation. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Parakh, S., and Atkin JD. (2015). Front. Cell Dev. Biol. 3; 30; Lumb, RA., and Bulleid, NJ. (2002). EMBO J. 21(24); 6763-6770). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |