Anti-14-3-3 β/α Antibody, clone 1D15 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1856 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from HeLa (Lane 1), SH-SY5Y (Lane 2), COS-1 (Lane 3) cells probed with Cat. No. ZRB1856, Anti-14-3-3 β/α, clone 1D15 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Rabbit Anti-Donkey IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates 14-3-3 β/α protein (~28 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of 14-3-3 β/α peptide (Run 1) or non-specific control peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1856, Anti-14-3-3 β/α, clone 1D15 ZooMAb® Rabbit Monoclonal. The binding of this antibody to 14-3-3 β/α peptide displayed a KD value of 2.3 x 10-6, whereas its binding to non-specific control peptide displayed a KD of 2.0 x 10-3.
ELISA Enhanced Validation - Recombinant Antibody Technology 14-3-3 β/α protein was analyzed using two-fold serial dilutions of Cat. No. ZRB1856, Anti-14-3-3 β/α, clone 1D15 ZooMAb® Rabbit Monoclonal. Plate was coated with 100 mL of recombinant 14-3-3 β/α protein (1 mg/mL). Protein was detected using alkaline phosphatase conjugated Goat Anti-Rabbit IgG (H&L) and pNPP substrate.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1D15, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt ~28.08 kDa |
| observed mol wt ~28 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, monkey |
| species reactivity (predicted by homology) | sheep, rat, bovine |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P31946  |
| Application: | Quality Control Testing Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected 14-3-3 β/a in HeLa cell lysate. Tested Applications Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected 14-3-3 β/a in SHSY5Y and COS-1 cell lysates. ELISA Analysis: 1.0 μg/mL from a representative lot detected recombinant 14-3-3 β/a. Affinity Binding Assay: A representative lot of this antibody bound 14-3-3 b/a peptide with a KD of 2.3 x 10-6 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-14-3-3 β/a, clone 1D15 ZooMAb®, Cat. No. ZRB1856, is a recombinant Rabbit monoclonal antibody that detects 14-3-3 β/a and is tested for use in Affinity Binding Assay, ELISA, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human 14-3-3 protein β/a. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1D15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects 14-3-3 protein β/a. It targets an epitope within 19 amino acids from the C-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | 14-3-3 protein β/a (UniProt: P31946; also known as Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1) is encoded by the YWHAB gene (Gene ID: 7529) in human. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and heterodimers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 protein beta/alpha serves as an adapter protein that has been implicated in the regulation of a large spectrum of both general and specialized signaling pathways. It binds to many partners, usually by recognition of a phosphoserine or phosphothreonine motif and its binding generally results in the modulation of the activity of the binding partner. The alpha, brain-specific form, differs from the beta form in its ability to be phosphorylated on serine 60 by protein kinase C delta in a sphingosine-dependent fashion. 14-3-3 β/a is shown to block the nuclear translocation of the phosphorylated form of SR protein-specific kinase 2 (SRPK2) and antagonize its stimulatory effect on cyclin D1 expression that results in blockage of SRPK2 elicited neuronal apoptosis. It also reported to serve as a negative regulator of osteogenesis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |