Anti-Lin28A Antibody, clone 1E3 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1918 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NCCIT cells was performed using a 1:1,000 dilution of Cat. No. ZRB1918, Anti-Lin28A, clone 1E3 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NCCIT cells was performed using a 1:1,000 dilution of Cat. No. ZRB1918, Anti-Lin28A, clone 1E3 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NCCIT cells was performed using a 1:1,000 dilution of Cat. No. ZRB1918, Anti-Lin28A, clone 1E3 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from NTERA2 (Lane 1), F9 (Lane 2), and H9 (Lane 3) cells were probed with Cat. No. ZRB1918, Anti-Lin28A, clone 1E3 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Lin28A (~28 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of non-specific control peptide (Run 1) or Lin28A peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1918, Anti-Lin28A, clone 1E3 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Lin28A peptide displayed a KD of 4.8 x 10-9 whereas binding to non-specific control peptide displayed a KD of 2.7 x 10-6.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million NTERA2 cells was performed using 0.1 of Cat. No. ZRB1918, Anti-Lin28A, clone 1E3 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1E3, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 22.74 kDa |
| observed mol wt ~28 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_078950  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | monkey, porcine |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9H9Z2 |
| Application: | Quality Control Testing Evaluated by Western Blotting in NTERA2 cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Lin28A in NTERA2 cell lysate. Tested applications Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Lin28A in F9 and H9 cell lysate. Flow Cytometry Analysis: 0.1 g from a representative lot detected Lin28A in one million NTERA2 cells. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lin28A in NCCIT cells. Affinity Binding Assay:: A representative lot of this antibody bound Lin28A with a KD of 4.8 x 10-9 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Lin-28A. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1E3 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Lin28A. It targets an epitope within 14 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Protein lin-28 homolog A (UniProt: Q9H9Z2; also known as Lin-28A, Zinc finger CCHC domain-containing protein 1) is encoded by the LIN28A (also known as CSDD1, LIN28, ZCCHC1) gene (Gene ID: 79727) in human. Lin28A is a monomeric RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency, and metabolism. Its expression is observed in embryonic stem cells, placenta, and testis. Lin28A is predominantly cytoplasmic, and it localizes to peri-endoplasmic reticulum regions and is detected in the microsomal fraction derived from rough endoplasmic reticulum (RER). Its shuttling from the nucleus to the cytoplasm requires RNA-binding. In the peri-endoplasmic reticulum region it binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, which reduces the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Lin28A is considered a translational enhancer that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. It is shown to be essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Lin28A levels are reported to be up-regulated in several primary tumors and is shown to facilitate cellular transformation in vitro. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jin, J., et al. (2011). Nucleic Acids Res. 39(9); 3724-3734; Heo, I., et al. (2008). Mol. Cell. 32(2); 276-284). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |