Discovery® RP-AmideC16 HPLC Column
SUPELCO/505013 - 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Product Type: Equipment/Book
| agency | suitable for USP L60 |
| application(s) | food and beverages |
| extent of labeling | 11% Carbon loading |
| feature | endcapped |
| impurities | <10 ppm metals |
| L × I.D. | 15 cm × 4.6 mm |
| manufacturer/tradename | Discovery® |
| matrix | silica gel, high purity, spherical base material |
| matrix active group | amide, alkyl phase |
| operating pH range | 2-8 |
| parameter | ≤70 °C temp. range |
| 400 bar pressure (5801 psi) | |
| particle size | 5 μm |
| pore size | 180 Å |
| Quality Level | 100  |
| separation technique | reversed phase |
| surface area | 200 m2/g |
| surface coverage | 2.6 μmol/m2 |
| technique(s) | HPLC: suitable |
| Application: | Discovery RP-AmideC16 HPLC Column may be used for the separation of: • Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD). • Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD). • Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection. • Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector. |
| Application: | Discovery® RP-AmideC16 HPLC column may be used for the separation of: • Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA). • Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design. • Hydrophilic bases by LC with principal component analysis (PCA). • 2,4-Dinitrophenylhydraz |
| Features and Benefits: | • Excellent retention and resolution of polar compounds • Unique selectivity compared to C18 • Excellent peak shape and efficiency • Less hydrophobic than C18 columns • Compatible with 100% aqueous mobile phases |
| General description: | Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group. |
| Legal Information: | Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41115700 |