Ascentis^® Express F5, 5 μm HPLC Column

SUPELCO/50631-U - 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Synonym: Core-shell (SPP) Fused Core PFP HPLC column

Product Type: Equipment/Book

Catalog Number	PKG Qty.	Price


45-50631-U	1 ea	$1000.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

agency	suitable for USP L43
application(s)	food and beverages
feature	endcapped
impurities	<5 ppm metals
L × I.D.	15 cm × 4.6 mm
manufacturer/tradename	Ascentis^®
material	stainless steel column
matrix	Fused-Core particle platform
	superficially porous particle
matrix active group	PFP (pentafluorophenyl) phase
operating pH	2-8
packaging	1 ea of
parameter	60 °C temp. range
	600 bar max. pressure (9000 psi)
particle size	5 μm
pore size	90 Å pore size
product line	Ascentis^®
Quality Level	100
separation technique	reversed phase
surface area	90 m²/g
technique(s)	HPLC: suitable
	LC/MS: suitable

Application:	Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employed the Ascentis^® Express F5, 5 µm HPLC column to determine lumacaftor and its degradation products in the presence of ivacaftor. The application of high-performance liquid chromatography ion trap time-of-flight mass spectrometry provided high sensitivity and resolution, showcasing the column′s efficacy in complex sample matrices (Özcan et al., 2023 ). Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: This research highlights the use of the Ascentis^® Express F5, 5 µm HPLC column in developing stability-indicating methods for tasimelteon. The column′s performance in both LC-MS/MS and LC-DAD methods ensured accurate and reliable identification of degradation products (Özcan et al., 2020 ). Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review discusses advancements in HPLC technology, including the Ascentis^® Express F5, 5 µm HPLC column. The article emphasizes the column′s capability in ultra-fast separations, providing enhanced efficiency and resolution in various analytical applications (Ali et al., 2012 ).
General description:	Ascentis^® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core^® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Legal Information:	Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information:	Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
Recommended products:	Discover LiChropur reagents ideal for HPLC or LC-MS analysis

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

41115700

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