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Ascentis® Express RP-Amide, 2 μm UHPLC Column

SUPELCO/51576-U - 2 μm particle size, L × I.D. 10 cm × 2.1 mm

Synonym: Core-shell (SPP) Fused Core polar embedded HPLC column

Product Type: Equipment/Book
Catalog Number PKG Qty. Price Quantity
45-51576-U 1 ea
 $1110.00
1/EA		 Add To Favorites

 

agency suitable for USP L60
application(s) food and beverages
feature endcapped
impurities <5 ppm (metals)
L × I.D. 10 cm × 2.1 mm
manufacturer/tradename Ascentis®
material stainless steel column
matrix Fused-Core particle platform
  superficially porous particle
matrix active group amide, alkyl phase
packaging 1 ea of
parameter 1000 bar max. pressure (14500 psi)
  60 °C temp. range
particle size 2 μm
pH 2-9
pore size 90 Å pore size
product line Ascentis®
Quality Level 100 
separation technique reversed phase
surface area 120 m2/g
technique(s) LC/MS: suitable
  UHPLC-MS: suitable
  UHPLC: suitable
Application:

  • A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study demonstrates the application of the Ascentis® Express RP-Amide, 2 μm UHPLC Column for analyzing complex mixtures of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The developed method was validated for precision, accuracy, and robustness, showing significant improvement in separation efficiency and analysis time compared to conventional columns. This method is particularly valuable for researchers focusing on phytochemical analysis and natural product chemistry (Fibigr et al., 2018 ).
General description: Ascentis® Express 2 μm RP-Amide is a high-speed, high-performance liquid chromatography column based on a new Fused-Core® particle design. The fused core partical provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 micron thick porous shell and the small overall particle size of 2-microns. The one-step bonded, end-capped, amide-based polar-embedded stationary phase of Ascentis Express 2 μm RP-Amide provides, a stable, reversed phase packing with decreased hydrophobic character. Ascentis Express 2 μm RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8.
Legal Information: Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information: Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
Recommended products: Discover LiChropur reagents ideal for HPLC  or LC-MS  analysis
UNSPSC 41115700

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