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Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

SUPELCO/53353-U - 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Synonym: Core-shell (SPP) Fused Core Phenyl HPLC column

Product Type: Equipment/Book

Catalog Number PKG Qty. Price Quantity
45-53353-U 1 ea
$1000.00
1/EA
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agency suitable for USP L11
application(s) food and beverages
feature endcapped
impurities <5 ppm metals
L × I.D. 15 cm × 4.6 mm
manufacturer/tradename Ascentis®
material stainless steel column
matrix Fused-Core particle platform
  superficially porous particle
matrix active group phenylhexyl phase
packaging 1 ea of
parameter 60 °C temp. range
  600 bar max. pressure (9000 psi)
particle size 2.7 μm
pH range 2-9
pore size 90 Å
product line Ascentis®
Quality Level 100 
separation technique reversed phase
surface area 135 m2/g
technique(s) HPLC: suitable
  LC/MS: suitable
  UHPLC-MS: suitable
  UHPLC: suitable
Application:
  • A Different Perspective on the Characterization of a New Degradation Product of Flibanserin With HPLC-DAD-ESI-IT-TOF-MSn and Its Pharmaceutical Formulation Analysis With Inter-Laboratory Comparison.: This study utilizes the Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC column to characterize degradation products of flibanserin, demonstrating its efficacy in high-resolution analytical applications (Geven et al., 2023 ).
  • HPLC with spectrophotometric or mass spectrometric detection for quantifying very-long chain fatty acids in human plasma and its association with cardiac risk factors.: The Ascentis® Express Phenyl-Hexyl column was employed for precise quantification of fatty acids, linking its use to critical clinical research on cardiac risk factors (Shrestha et al., 2021 ).
  • A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers.: This research highlights the efficiency of the Ascentis® Express Phenyl-Hexyl column in detecting contaminants in beverages, emphasizing its role in food safety analysis (Lhotska et al., 2016 ).
  • A study of retention characteristics and quality control of nutraceuticals containing resveratrol and polydatin using fused-core column chromatography.: This study explores the retention characteristics of nutraceuticals using the Ascentis® Express Phenyl-Hexyl column, showcasing its utility in quality control of health supplements (Fibigr et al., 2016 ).
  • An on-line SPE-HPLC method for effective sample preconcentration and determination of fenoxycarb and cis, trans-permethrin in surface waters.: The Ascentis® Express Phenyl-Hexyl column was integral in preconcentrating and analyzing pesticide residues in environmental samples, underscoring its environmental analytical applications (Satinsky et al., 2015 ).
General description: The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.
Legal Information: Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
UNSPSC 41115700

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