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Ascentis® Express F5, 2.7 μm HPLC Column

SUPELCO/53591-U - 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Synonym: Core-shell (SPP) Fused Core PFP HPLC column

Product Type: Equipment/Book

Catalog Number PKG Qty. Price Quantity
45-53591-U 1 ea
$1000.00
1/EA
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agency suitable for USP L43
application(s) food and beverages
feature endcapped
impurities <5 ppm metals
L × I.D. 15 cm × 4.6 mm
manufacturer/tradename Ascentis®
material stainless steel column
matrix Fused-Core particle platform
  superficially porous particle
matrix active group PFP (pentafluorophenyl) phase
operating pH 2-8
packaging 1 ea of
parameter 60 °C temp. range
  600 bar max. pressure (9000 psi)
particle size 2.7 μm
pore size 90 Å pore size
product line Ascentis®
Quality Level 100 
separation technique reversed phase
surface area 135 m2/g
technique(s) HPLC: suitable
  LC/MS: suitable
  UHPLC-MS: suitable
  UHPLC: suitable
Application:

  • Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study demonstrates the use of high-performance liquid chromatography (HPLC) with ion trap time-of-flight mass spectrometry for the determination of lumacaftor in the presence of ivacaftor. The research identifies five novel degradation products, showcasing the advanced analytical capabilities of the Ascentis® Express F5, 2.7 µm HPLC Column for complex mixtures and degradation studies (Özcan et al., 2023 ).

  • Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: This paper details the development of stability-indicating methods using LC-MS/MS and LC-DAD for the determination of tasimelteon. It highlights the robustness and sensitivity of these methods in identifying novel degradation products, with the Ascentis® Express F5 column playing a crucial role in separation efficiency and stability analysis (Özcan et al., 2020 ).

  • Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review article discusses recent advancements in ultra-fast HPLC, focusing on new generation superficially porous silica columns, including the Ascentis® Express F5, 2.7 µm HPLC Column. It provides insights into the enhanced performance, speed, and efficiency of these columns in high-throughput analytical applications (Ali et al., 2012 ).

General description: The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Legal Information: Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
UNSPSC 41115700

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