apHera™ NH2 HPLC Column
SUPELCO/56401AST - 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Synonym: Amino HPLC Column
Product Type: Equipment/Book
| agency | suitable for USP L82 |
| application(s) | food and beverages |
| L × I.D. | 15 cm × 4.6 mm |
| manufacturer/tradename | apHera™ |
| material | stainless steel column |
| matrix | polymeric |
| matrix active group | amino phase |
| operating pH | 1-14 |
| packaging | pkg of 1 ea |
| parameter | 147 bar pressure (2133 psi) |
| 4-50 °C temperature | |
| particle size | 5 μm |
| pore size | 300 Å |
| product line | apHera™ |
| Quality Level | 100  |
| separation technique | hydrophilic interaction (HILIC) |
| normal phase | |
| technique(s) | HPLC: suitable |
| LC/MS: suitable |
| General description: | apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column. Features of Amino apHera™ Columns • High efficiency for carbohydrate analysis • Ideal for basic conditions/amphoteric detection • High selectivity mono- to oligosaccharides • pH range 2-13 • 300 angstrom pore size • Stable, predictable retention • Long column life |
| Legal Information: | apHera is a trademark of Sigma-Aldrich Co. LLC |
| Recommended products: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| UNSPSC | 41115700 |