Discovery^® BIO Wide Pore C18 HPLC Column

SUPELCO/567205-U - 3 μm particle size, L × I.D. 15 cm × 4.6 mm

Synonym: Wide Pore C18 Column

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-567205-U	1 ea	$1210.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

agency	suitable for USP L1
extent of labeling	9.2% Carbon loading
feature	endcapped
L × I.D.	15 cm × 4.6 mm
manufacturer/tradename	Discovery^®
material	stainless steel column
matrix	silica particle platform
	fully porous particle
matrix active group	C18 (octadecyl) phase
operating pH	2-8
packaging	1 ea of
parameter	0-70 °C temperature
	400 bar pressure (5801 psi)
particle size	3 μm
pore size	300 Å
product line	Discovery^®
Quality Level	100
separation technique	reversed phase
surface area	100 m²/g
surface coverage	3.6 μmol/m²
technique(s)	HPLC: suitable
	LC/MS: suitable

Application:	Kinetic performance comparison of superficially porous, fully porous and monolithic reversed-phase columns by gradient kinetic plots for the separation of protein biopharmaceuticals.: This study provides a detailed kinetic performance comparison of different types of reversed-phase HPLC columns, including the Discovery^® BIO Wide Pore C18 HPLC Column. The research highlights its efficiency and resolution in separating complex protein biopharmaceuticals, making it a valuable tool for analytical chemists in biopharmaceutical analysis (Jaag et al., 2022 ). RP-LC x RP-LC analysis of a tryptic digest using a combination of totally porous and partially porous stationary phases.: This paper investigates the use of reverse-phase liquid chromatography (RP-LC) with both totally porous and partially porous stationary phases, including the BIO Wide Pore C18 HPLC Column, to analyze tryptic digests. The findings demonstrate its robustness and high resolution in proteomics applications, enabling precise peptide mapping and protein characterization (Mondello et al., 2010 ).
General description:	Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
Legal Information:	Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Recommended products:	Discover LiChropur reagents ideal for HPLC or LC-MS analysis

WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

41115700

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