Discovery® BIO Wide Pore C18 HPLC Column
SUPELCO/567212-U - 10 μm particle size, L × I.D. 25 cm × 21.2 mm
Product Type: Chemical
| agency | suitable for USP L1 |
| extent of labeling | 9.2% Carbon loading |
| feature | endcapped |
| L × I.D. | 25 cm × 21.2 mm |
| manufacturer/tradename | Discovery® |
| material | stainless steel column |
| matrix | silica particle platform |
| fully porous particle | |
| matrix active group | C18 (octadecyl) phase |
| operating pH | 2-8 |
| packaging | 1 ea of |
| parameter | 0-70 °C temperature |
| 400 bar pressure (5801 psi) | |
| particle size | 10 μm |
| pore size | 300 Å |
| product line | Discovery® |
| Quality Level | 100  |
| separation technique | reversed phase |
| surface area | 100 m2/g |
| surface coverage | 3.6 μmol/m2 |
| technique(s) | HPLC: suitable |
| LC/MS: suitable |
| General description: | Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal. |
| Legal Information: | Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Recommended products: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| UNSPSC | 41115700 |