Discovery® BIO Wide Pore C5 HPLC Column
SUPELCO/567229-U - 3 μm particle size, L × I.D. 5 cm × 4.6 mm
Product Type: Equipment/Book
| extent of labeling | 3.5% Carbon loading |
| feature | endcapped |
| L × I.D. | 5 cm × 4.6 mm |
| manufacturer/tradename | Discovery® |
| material | stainless steel column |
| matrix | silica particle platform |
| fully porous particle | |
| matrix active group | C5 (pentyl) phase |
| operating pH | 2-8 |
| packaging | 1 ea of |
| parameter | 0-70 °C temperature |
| 400 bar pressure (5801 psi) | |
| particle size | 3 μm |
| pore size | 300 Å |
| product line | Discovery® |
| Quality Level | 100  |
| separation technique | reversed phase |
| surface area | 100 m2/g |
| surface coverage | 4.5 μmol/m2 |
| technique(s) | HPLC: suitable |
| LC/MS: suitable |
| General description: | Discovery BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC. Long-chain phases, like C8 or C18, are often too hydrophobic for proteins and can cause excessively long retention time or even irreversible binding to the column. For this reason short-chain phases, typically C3 or C4, are often used for RP-HPLC of proteins. However, these short-chain phases are susceptible to hydrolysis, resulting in short column lifetime, especially at low pH. The Discovery BIO Wide Pore C5 gives elution order similar to a conventional C4, yet has enhanced pH stability for longer column lifetime. Generally, higher efficiency separations are achievable on the Discovery BIO Wide Pore C5 because of the improvements we have made to the silica and bonded-phase chemistry. |
| Legal Information: | Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Recommended products: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| UNSPSC | 41115700 |