Discovery® HS F5 HPLC Column
SUPELCO/567512-U - 5 μm particle size, L × I.D. 25 cm × 2.1 mm
Product Type: Equipment/Book
| agency | suitable for USP L43 |
| application(s) | food and beverages |
| extent of labeling | 12% Carbon loading |
| feature | endcapped |
| impurities | <10 ppm metals |
| L × I.D. | 25 cm × 2.1 mm |
| manufacturer/tradename | Discovery® |
| material | stainless steel column |
| matrix | silica gel, high purity, spherical particle platform |
| fully porous particle | |
| matrix active group | PFP (pentafluorophenyl) phase |
| operating pH range | 2-8 |
| packaging | 1 ea of |
| parameter | ≤70 °C temp. range |
| 400 bar pressure (5801 psi) | |
| particle size | 5 μm |
| pore size | 120 Å |
| product line | Discovery® |
| Quality Level | 100  |
| separation technique | reversed phase |
| surface area | 300 m2/g |
| surface coverage | 4 μmol/m2 |
| technique(s) | HPLC: suitable |
| LC/MS: suitable |
| Features and Benefits: | • Unique selectivity • Similar retention to C18 (sometimes requires stronger mobile phase) • Excellent peak shape • Stable, low-bleed LC-MS separations • Scalable separations from 3 to 10μm particle sizes |
| General description: | Guidelines for transferring a C18 method to Discovery® HS F5: Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent. |
| General description: | The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable. |
| Legal Information: | Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Recommended products: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| UNSPSC | 41115700 |