Discovery^® HS F5 HPLC Column

SUPELCO/567513-U - 5 μm particle size, L × I.D. 5 cm × 4.6 mm

Synonym: Discovery Pentafluorophenyl PFP HPLC Column

Product Type: Equipment/Book

Catalog Number	PKG Qty.	Price


45-567513-U	1 ea	$623.00 1/EA	Add To Favorites

Properties
Descriptions
Basic Data

agency	suitable for USP L43
application(s)	food and beverages
extent of labeling	12% Carbon loading
feature	endcapped
impurities	<10 ppm metals
L × I.D.	5 cm × 4.6 mm
manufacturer/tradename	Discovery^®
material	stainless steel column
matrix	silica gel, high purity, spherical particle platform
	fully porous particle
matrix active group	PFP (pentafluorophenyl) phase
operating pH range	2-8
packaging	1 ea of
parameter	≤70 °C temp. range
	400 bar pressure (5801 psi)
particle size	5 μm
pore size	120 Å
product line	Discovery^®
Quality Level	100
separation technique	reversed phase
surface area	300 m²/g
surface coverage	4 μmol/m²
technique(s)	HPLC: suitable
	LC/MS: suitable

Application:	Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring.: This study utilizes the Discovery^® HS F5 HPLC Column to develop an HPLC-UV method for the simultaneous determination of phenylalanine and tyrosine in blood, significantly advancing the diagnostic and monitoring capabilities for phenylketonuria (Rabti et al., 2024 ). A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities.: Demonstrates the application of the Discovery^® HS F5 HPLC Column in optimizing sample preparation techniques for the detection of pharmaceuticals and personal care products in environmental samples, enhancing the analysis of diverse hydrophilic compounds (Portillo-Castillo et al., 2021 ). "Heart-cut" bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity and brain response to chiral drug candidates targeting the central nervous system.: Utilizes the Discovery^® HS F5 HPLC Column in a novel bidimensional chromatographic setup to study the stereoselective metabolism of CNS-targeting chiral drugs, contributing to a deeper understanding of their pharmacological profiles (Battisti et al., 2016 ). HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776).: This research employs the Discovery^® HS F5 HPLC Column for developing an HPLC method to identify and characterize impurities in the antitumor drug LMP776, ensuring drug purity and compliance with regulatory standards (Wang et al., 2016 ). Development studies on determination of preservatives decomposition products.: Showcases the application of the Discovery^® HS F5 HPLC Column in the analysis of decomposition products of preservatives, enhancing the safety assessments of food and pharmaceutical products (Zabrzewska et al., 2014 ).
Features and Benefits:	• Unique selectivity • Similar retention to C18 (sometimes requires stronger mobile phase) • Excellent peak shape • Stable, low-bleed LC-MS separations • Scalable separations from 3 to 10μm particle sizes
General description:	Guidelines for transferring a C18 method to Discovery® HS F5: Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
General description:	The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Legal Information:	Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Recommended products:	Discover LiChropur reagents ideal for HPLC or LC-MS analysis

UNSPSC

41115700

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