Discovery^® HS F5 HPLC Column

SUPELCO/567516-U - 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Synonym: Discovery Pentafluorophenyl PFP HPLC Column

Product Type: Equipment/Book

Catalog Number	PKG Qty.	Price


45-567516-U	1 ea	$926.00 1/EA	Add To Favorites

Properties
Descriptions
Basic Data

agency	suitable for USP L43
application(s)	food and beverages
extent of labeling	12% Carbon loading
feature	endcapped
impurities	<10 ppm metals
L × I.D.	15 cm × 4.6 mm
manufacturer/tradename	Discovery^®
material	stainless steel column
matrix	silica gel, high purity, spherical particle platform
	fully porous particle
matrix active group	PFP (pentafluorophenyl) phase
operating pH range	2-8
packaging	1 ea of
parameter	≤70 °C temp. range
	400 bar pressure (5801 psi)
particle size	5 μm
pore size	120 Å
product line	Discovery^®
Quality Level	100
separation technique	reversed phase
surface area	300 m²/g
surface coverage	4 μmol/m²
technique(s)	HPLC: suitable
	LC/MS: suitable

Application:	Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.: This study leverages the Discovery^® HS F5 HPLC Column for advanced arsenic speciation in rice, enhancing food safety analysis and ensuring compliance with health regulations (Baba et al., 2014 ). High-performance liquid chromatographic determination of tiapride and its phase I metabolite in blood plasma using tandem UV photodiode-array and fluorescence detection.: Demonstrates the versatility of the Discovery^® HS F5 HPLC Column in the sensitive and simultaneous detection of tiapride and its metabolites, contributing to therapeutic drug monitoring and pharmacokinetic studies (Nobilis et al., 2011 ). Simultaneous quantification of nicotine and metabolites in rat brain by liquid chromatography-tandem mass spectrometry.: Utilizes the Discovery^® HS F5 HPLC Column to accurately measure nicotine and its metabolites in rat brain, providing crucial data for studies on addiction and neuropharmacology (Vieira-Brock et al., 2011 ). Biomarker discovery in biological specimens (plasma, hair, liver and kidney) of diabetic mice based upon metabolite profiling using ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry.: This research uses the Discovery^® HS F5 HPLC Column to identify biomarkers in various biological samples from diabetic mice, enhancing our understanding of diabetes at the molecular level (Tsutsui et al., 2011 ). Practical analytical approach for the identification of biomarker candidates in prediabetic state based upon metabonomic study by ultraperformance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry.: Employs the Discovery^® HS F5 HPLC Column in a metabolomic approach to identify early biomarkers of diabetes, advancing preventive medicine and metabolic research (Tsutsui et al., 2010 ).
Features and Benefits:	• Unique selectivity • Similar retention to C18 (sometimes requires stronger mobile phase) • Excellent peak shape • Stable, low-bleed LC-MS separations • Scalable separations from 3 to 10μm particle sizes
General description:	Guidelines for transferring a C18 method to Discovery® HS F5: Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
General description:	The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Legal Information:	Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Recommended products:	Discover LiChropur reagents ideal for HPLC or LC-MS analysis

UNSPSC

41115700

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