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Discovery® HS F5 HPLC Column

SUPELCO/567516-U - 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Synonym: Discovery Pentafluorophenyl PFP HPLC Column

Product Type: Equipment/Book

Catalog Number PKG Qty. Price Quantity
45-567516-U 1 ea
$926.00
1/EA
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agency suitable for USP L43
application(s) food and beverages
extent of labeling 12% Carbon loading
feature endcapped
impurities <10 ppm metals
L × I.D. 15 cm × 4.6 mm
manufacturer/tradename Discovery®
material stainless steel column
matrix silica gel, high purity, spherical particle platform
  fully porous particle
matrix active group PFP (pentafluorophenyl) phase
operating pH range 2-8
packaging 1 ea of
parameter ≤70 °C temp. range
  400 bar pressure (5801 psi)
particle size 5 μm
pore size 120 Å
product line Discovery®
Quality Level 100 
separation technique reversed phase
surface area 300 m2/g
surface coverage 4 μmol/m2
technique(s) HPLC: suitable
  LC/MS: suitable
Application:

  • Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.: This study leverages the Discovery® HS F5 HPLC Column for advanced arsenic speciation in rice, enhancing food safety analysis and ensuring compliance with health regulations (Baba et al., 2014 ).

  • High-performance liquid chromatographic determination of tiapride and its phase I metabolite in blood plasma using tandem UV photodiode-array and fluorescence detection.: Demonstrates the versatility of the Discovery® HS F5 HPLC Column in the sensitive and simultaneous detection of tiapride and its metabolites, contributing to therapeutic drug monitoring and pharmacokinetic studies (Nobilis et al., 2011 ).

  • Simultaneous quantification of nicotine and metabolites in rat brain by liquid chromatography-tandem mass spectrometry.: Utilizes the Discovery® HS F5 HPLC Column to accurately measure nicotine and its metabolites in rat brain, providing crucial data for studies on addiction and neuropharmacology (Vieira-Brock et al., 2011 ).

  • Biomarker discovery in biological specimens (plasma, hair, liver and kidney) of diabetic mice based upon metabolite profiling using ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry.: This research uses the Discovery® HS F5 HPLC Column to identify biomarkers in various biological samples from diabetic mice, enhancing our understanding of diabetes at the molecular level (Tsutsui et al., 2011 ).

  • Practical analytical approach for the identification of biomarker candidates in prediabetic state based upon metabonomic study by ultraperformance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry.: Employs the Discovery® HS F5 HPLC Column in a metabolomic approach to identify early biomarkers of diabetes, advancing preventive medicine and metabolic research (Tsutsui et al., 2010 ).

Features and Benefits: • Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes
General description: Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
General description: The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Legal Information: Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Recommended products: Discover LiChropur reagents ideal for HPLC  or LC-MS  analysis
UNSPSC 41115700

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