Ascentis^® C18 HPLC Column

SUPELCO/581322-U - 3 μm particle size, L × I.D. 15 cm × 4.6 mm

Synonym: Ascentis RP18 HPLC Column

Product Type: Equipment/Book

Catalog Number	PKG Qty.	Price


45-581322-U	1 ea	$1090.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

agency	suitable for USP L1 (Similar to Phenomenex Luna C18)
application(s)	food and beverages
extent of labeling	25% Carbon loading
feature	endcapped
impurities	<5 ppm metals
L × I.D.	15 cm × 4.6 mm
manufacturer/tradename	Ascentis^®
material	stainless steel column
matrix	fully porous particle
	silica gel high purity, spherical
matrix active group	C18 (octadecyl) phase
operating pH range	2-8
packaging	1 ea of
parameter	≤70 °C temp. range
	400 bar pressure (5801 psi)
particle size	3 μm
pore size	100 Å
product line	Ascentis^®
Quality Level	100
separation technique	reversed phase
surface area	450 m²/g
surface coverage	3.7 μmol/m²
technique(s)	HPLC: suitable
	LC/MS: suitable

Application:	A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals: This paper details the application of Ascentis^® C18 columns in a clinical setting to measure cortisol levels in urine. The method provides a fast, accurate, and reliable way to assess patient hormonal profiles, critical for diagnosing endocrine disorders (Zhao et al., 2022 ). A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS: Utilizing Ascentis^® C18, this study compares different analytical methods for quantifying ginsenosides. It showcases the column′s adaptability and precision in natural product research, essential for ensuring the quality and efficacy of herbal medicines (Hsu et al., 2022 ). Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Highlighting the Ascentis^® C18′s effectiveness in pharmaceutical formulations, this research demonstrates its capacity to analyze complex drug delivery systems, contributing to the development of more effective therapeutic solutions (Brugnera et al., 2022 ). Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method: This publication illustrates the application of the Ascentis^® C18 in veterinary medicine, providing crucial insights into drug stability and composition which are vital for regulatory compliance and animal safety (Padivitage et al., 2022 ).
Features and Benefits:	• Excellent retention • Symmetric peak shape • High reproducibility • Complete LC-MS compatibility
General description:	The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide. Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
Legal Information:	Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Recommended products:	Discover LiChropur reagents ideal for HPLC or LC-MS analysis

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

41115700

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