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Ascentis® C18 HPLC Column

SUPELCO/581324-U - 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Synonym: Ascentis RP18 HPLC Column

Product Type: Equipment/Book
Catalog Number PKG Qty. Price Quantity
45-581324-U 1 ea
 $912.00
1/EA		 Add To Favorites

 

agency suitable for USP L1 (Similar to Phenomenex Luna C18)
application(s) food and beverages
extent of labeling 25% Carbon loading
feature endcapped
impurities <5 ppm metals
L × I.D. 15 cm × 4.6 mm
manufacturer/tradename Ascentis®
material stainless steel column
matrix fully porous particle
  silica gel high purity, spherical
matrix active group C18 (octadecyl) phase
operating pH range 2-8
packaging 1 ea of
parameter ≤70 °C temp. range
  400 bar pressure (5801 psi)
particle size 5 μm
pore size 100 Å
product line Ascentis®
Quality Level 100 
separation technique reversed phase
surface area 450 m2/g
surface coverage 3.7 μmol/m2
technique(s) HPLC: suitable
  LC/MS: suitable
Application:

  • Preparation of polar embedded C18 stationary phase for efficient separation of peptides and proteins in high performance liquid chromatography.: This study details the development of a polar embedded C18 stationary phase, demonstrating its effectiveness in separating peptides and proteins. The Ascentis® C18 HPLC Column was utilized for its high separation efficiency, crucial for proteomics and biopharmaceutical applications (Ali et al., 2022 ).

  • A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals.: Researchers used the Ascentis® C18 HPLC Column to develop a sensitive method for quantifying urinary 18-hydroxycortisol. This method aids in clinical diagnostics and endocrinological studies by providing accurate reference intervals (Zhao et al., 2022 ).

  • A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: This comparative analysis utilized the Ascentis® C18 HPLC Column to separate and identify ginsenosides in American ginseng. The study highlights the column’s application in natural product research and quality control in the pharmaceutical industry (Hsu et al., 2022 ).

  • Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: This research validates an HPLC method using the Ascentis® C18 Column to simultaneously quantify dexamethasone and melatonin in PLGA microspheres, offering a robust tool for drug delivery studies (Brugnera et al., 2022 ).

  • Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: The Ascentis® C18 HPLC Column was employed to develop a stability-indicating method for analyzing ivermectin and clorsulon in pharmaceutical formulations, emphasizing its role in quality control and regulatory compliance (Padivitage et al., 2022 ).
Features and Benefits: • Excellent retention
• Symmetric peak shape
• High reproducibility
• Complete LC-MS compatibility
General description: The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
Legal Information: Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Recommended products: Discover LiChropur reagents ideal for HPLC  or LC-MS  analysis
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
UNSPSC 41115700

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