Ascentis^® C18 HPLC Column

SUPELCO/581325-U - 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Synonym: Ascentis RP18 HPLC Column

Product Type: Equipment/Book

Catalog Number	PKG Qty.	Price


45-581325-U	1 ea	$1000.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

agency	suitable for USP L1 (Similar to Phenomenex Luna C18)
application(s)	food and beverages
extent of labeling	25% Carbon loading
feature	endcapped
impurities	<5 ppm metals
L × I.D.	25 cm × 4.6 mm
manufacturer/tradename	Ascentis^®
material	stainless steel column
matrix	fully porous particle
	silica gel high purity, spherical
matrix active group	C18 (octadecyl) phase
operating pH range	2-8
packaging	1 ea of
parameter	≤70 °C temp. range
	400 bar pressure (5801 psi)
particle size	5 μm
pore size	100 Å
product line	Ascentis^®
Quality Level	100
separation technique	reversed phase
surface area	450 m²/g
surface coverage	3.7 μmol/m²
technique(s)	HPLC: suitable
	LC/MS: suitable

Application:	Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: This study utilized the Ascentis^® C18 HPLC column for the separation of proteotypic tryptic peptides in the identification of insulin analogues, highlighting its application in targeted mass spectrometry for proteomic analysis (Qasem RJ et al., 2019 ). Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation.: This research developed and validated an HPLC method using the Ascentis^® C18 HPLC column for the simultaneous assay of multiple compounds, demonstrating its robustness in stability testing of pharmaceutical formulations (Byrne J et al., 2014 ). HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: The study employed the Ascentis^® C18 HPLC column for metabolite profiling of propolis extracts using HPLC-DAD and HPLC-ESI-MS/MS, showcasing its effectiveness in complex natural product analysis (Pellati F et al., 2011 ). Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: This article compared different HPLC column technologies, including the Ascentis^® C18, highlighting its performance in fast separations critical for pharmaceutical process development (Abrahim A et al., 2010 ).
Features and Benefits:	• Excellent retention • Symmetric peak shape • High reproducibility • Complete LC-MS compatibility
General description:	The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide. Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
Legal Information:	Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Recommended products:	Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Hazard Codes	Xi
Risk Statements	36
Safety Statements	26
RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

UNSPSC

41115700

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