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Ascentis® Phenyl HPLC Column

SUPELCO/581616-U - 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Synonym: Phenyl HPLC Column

Product Type: Equipment/Book

Catalog Number PKG Qty. Price Quantity
45-581616-U 1 ea
$1090.00
1/EA
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agency suitable for USP L11
application(s) food and beverages
extent of labeling 19% Carbon loading
feature endcapped
impurities <5 ppm metals
L × I.D. 15 cm × 4.6 mm
manufacturer/tradename Ascentis®
material stainless steel column
matrix fully porous particle
  silica gel high purity, spherical
matrix active group phenyl phase
operating pH range 2-8
packaging 1 ea of
parameter ≤70 °C temp. range
  400 bar pressure (5801 psi)
particle size 5 μm
pore size 100 Å
product line Ascentis®
Quality Level 100 
separation technique reversed phase
surface area 450 m2/g
surface coverage 5.2 μmol/m2
technique(s) HPLC: suitable
  LC/MS: suitable
Application:

  • A Different Perspective on the Characterization of a New Degradation Product of Flibanserin With HPLC-DAD-ESI-IT-TOF-MSn and Its Pharmaceutical Formulation Analysis With Inter-Laboratory Comparison: This study highlights the advanced chromatographic techniques used to characterize pharmaceutical compounds, demonstrating the importance of accurate and detailed analysis in pharmaceutical research. The use of the Ascentis® Phenyl HPLC column facilitates high-resolution separations, essential for identifying degradation products in complex mixtures (Geven et al., 2023 ).

  • HPLC with Spectrophotometric or Mass Spectrometric Detection for Quantifying Very-Long Chain Fatty Acids in Human Plasma and Its Association with Cardiac Risk Factors: This paper discusses the role of high-performance liquid chromatography (HPLC) in clinical settings, particularly for the assessment of biomarkers related to heart disease. Techniques involving the Ascentis® Phenyl HPLC column are employed to achieve precise and reliable quantification of fatty acids, critical for clinical diagnostics (Shrestha et al., 2021 ).

  • A Fully Automated and Fast Method Using Direct Sample Injection Combined with Fused-Core Column On-Line SPE-HPLC for Determination of Ochratoxin A and Citrinin in Lager Beers: This study introduces an automated method for toxin analysis in beverages, utilizing the Ascentis® Phenyl HPLC column. The approach enhances sensitivity and throughput, showcasing the column′s efficacy in food safety applications (Lhotská et al., 2016 ).

  • A Study of Retention Characteristics and Quality Control of Nutraceuticals Containing Resveratrol and Polydatin Using Fused-Core Column Chromatography: The research focuses on nutraceuticals, where the Ascentis® Phenyl HPLC column′s selectivity plays a pivotal role in segregating and analyzing health-promoting compounds, thereby ensuring product quality and compliance with industry standards (Fibigr et al., 2016 ).

  • An On-Line SPE-HPLC Method for Effective Sample Preconcentration and Determination of Fenoxycarb and Cis, Trans-Permethrin in Surface Waters: This publication illustrates the application of the Ascentis® Phenyl HPLC column in environmental analysis, particularly for the sensitive detection of pesticides in water bodies. The column’s capabilities help in tracing low-level contaminants, critical for environmental monitoring and protection (Šatínský et al., 2015 ).

Features and Benefits: • 100% Aqueous Compatible
• ANP/HILIC and reversed-phase
• Low UV/MS bleed for gradient applications
• Alternate selectivity
General description: The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis Phenyl provides superior separations in reversed-phase mode including 100% aqueous conditions. It may also be used in HILIC/ANP (aqueous normal phase) mode and shows low UV/MS bleed for gradient applications.
Legal Information: Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Recommended products: Discover LiChropur reagents ideal for HPLC  or LC-MS  analysis
UNSPSC 41115700

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