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CHIRALPAK® HSA HPLC Column

SUPELCO/58430AST - 5 μm particle size, L × I.D. 10 cm × 2.1 mm

Product Type: Equipment/Book

Catalog Number PKG Qty. Price Quantity
45-58430AST 1 ea
$3750.00
1/EA
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agency suitable for USP L79
L × I.D. 10 cm × 2.1 mm
manufacturer/tradename CHIRALPAK®
material stainless steel column
matrix fully porous particle
matrix active group human serum albumin phase
operating pH 2-8
packaging pkg of 1 ea
parameter 137 bar pressure (2000 psi)
  20-30 °C temperature
  40 °C max. temp.
particle size 5 μm
product line CHIRALPAK®
Quality Level 100 
separation technique chiral
technique(s) HPLC: suitable
General description: CHIRALPAK HSA, which uses human serum albumin as the chiral selector, is highly selective for acidic racemates, preferably weak and strong acids, zwitterionic and non-protolytic (neutral) compounds. Phosphate buffers (normally 0.01-0.1M, pH 5-7) with addition of organic modifiers are used as mobile phases. Enantioselectivity and retention can be regulated by changing the mobile phase composition. However, the primary use of CHIRALPAK HSA is for fast drug/protein binding studies (1). To calculate the % protein binding, measure the retention time of an unretained compound (t0) and the compound of interest (tr) on the CHIRALPAK HSA column. Then use the capacity factor equation:
k = (tr - t0)/tr
to calculate the % protein binding (P):
P = 100k/(k+1)

Different types of mobile phases can be used. A mobile phase consisting of 6% 2-propanol in 20 mM potassium phosphate buffer, pH 7.0 gives data in good agreement with literature data. The mobile phase conditions should be chosen to suit the drugs to be tested, i.e., for high protein binding drugs a mobile phase with higher eluting strength might be needed in order to reduce retention times.

(1) Goodman, A.; Gilman, A.G. The Pharmacological Basis of Therapeutics, 9th Edition, McGraw-Hill: New York, 1996; pp 1712-1792.
Legal Information: CHIRALPAK is a registered trademark of Daicel Corp.
Recommended products: Discover LiChropur reagents ideal for HPLC  or LC-MS  analysis
UNSPSC 41115700

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